Correlational interdependencies between the sorbitol content and "classical" biochemical parameters characterizing the functional state of the liver in cattle

The article presents the results of a study of correlations between the clearance of exogenous sorbitol and the activity of traditional liver enzymes (ALT, AST, ALP) and the level of total bilirubin in clinically healthy cattle. Statistically significant positive correlations have been established between the concentration of sorbitol in blood plasma and the level of AST and alkaline phosphatase, which indicates the promising use of the sorbitol clearance test as an early marker of liver functional disorders. The data obtained substantiate the need for further research to develop new means of preclinical diagnosis of hepatopathy in productive animals. According to the results of the study, there was no statistically significant correlation between the level of sorbitol and the activity of ALT, as well as the concentration of total bilirubin. This may be due to the greater organ specificity of AST for the liver of cattle compared to ALT, as well as the fact that bilirubin levels increase with more serious and pronounced pathologies. The conducted study established the presence of statistically significant strong positive correlations between the concentration of exogenous sorbitol after its loading administration and the activity of key "classical" liver enzymes – AST and ALP in clinically healthy cattle. This indicates that a decrease in the metabolic clearance of sorbitol is closely associated with the initial pathobiochemical shifts characteristic of hepatocyte damage and cholestasis. The results of this study confirm the prospects of using the sorbitol clearance test as a highly sensitive functional test for preclinical diagnosis of liver disorders in cattle.

1. Baymishev H.B., Idrisov R.R. Liver diseases in highly productive cows: diagnosis and prevention // Veterinary medicine. 2020. No. 5. pp. 3-8.

2. Analysis of the causes of the disposal of beef cattle / V. A. Mishchenko, A.V. Mishchenko, V. V. Dumova, O. Yu. Chernykh // Veterinary medicine of Kuban. - 2014. – No. 3. – pp. 19-22.

3. Tumilovich, G. A. Methods of lifetime diagnosis of liver pathology in cattle with metabolic diseases / G. A. Tumilovich, D. N. Kharitonik // Agrarian science for agriculture: Proceedings of the XVI International Scientific and Practical Conference in 2 volumes, Barnaul, February 09-10, 2021. Volume 2. Barnaul: Altai State Agrarian University, 2021. pp. 199-201.

4. Abramov A. A., Rogaleva E. V., Semenenko M. P. Investigation of the preventive effect of philokvertin in hepatopathy of dairy cattle in dairy farm conditions // Collection of scientific papers of the Krasnodar Scientific Center for Animal Science and Veterinary Medicine. – 2022. – Vol. 11, No. 1. – pp. 253-256. – DOI 10.48612/sbornik2022-1-62 .

5. Markers of endogenous intoxication in dairy cattle hepatopathies / E. V. Kuzminova, A. A. Abramov, M. P. Semenenko [et al.]. – Krasnodar: Krasnodar Scientific Center for Animal Science and Veterinary Medicine, 2022. – 45 p. – ISBN 978-5-906643-46 -9. – DOI 10.48612/monograph-2023-7.

6. Serova, E. A. Diagnostics of liver diseases in cows / E. A. Serova, A. A. Loginova // Young researchers of agro–industrial and forestry complexes - by region, Vologda, April 03, 2025. Vologda-Molochnoye: Vologda State Agricultural Academy, 2025, pp. 128-133.

7. Golodyaeva, M. S. Early diagnosis of the biochemical status in first-calf cows with hepatosis / M. S. Golodyaeva, A. Ya. Batrakov // Issues of regulatory regulation in veterinary medicine. - 2018. – No. 4. – pp. 126- 128.

8. Expert systems in improving the effectiveness of liver disease diagnosis in animals / M. P. Semenenko, E. V. Kuzminova, S. I. Kononenko [et al.] // Veterinary medicine, animal science and biotechnology. - 2018. – No. 11. – pp. 62-68.

9. Parrah J.D., Moulvi B.A., Gani T.A., et al. Significance of Load Tests in Diagnosis of Liver Disorders in Bovines: A Review // Veterinary Research. – 2013. – Vol. 6(2). – P. 30-35.

10. Wimberley, P D et al. “International Federation of Clinical Chemistry (IFCC): Scientific Division, Committee on pH, Blood Gases and Electrolytes: Guidelines for Transcutaneouspo(2)andpco(2) Measurement.” The Journal of automatic chemistry vol. 11,5 (1989): 235-9. doi:10.1155/S1463924689000465

11. Kapelkin, A.M. Hematological and biochemical status of black-and-white cows during lactation / A.M. Kapelkin, N. A. Dudchenko, A. A. Tomilov // Collection of scientific papers of the All-Russian Scientific Research Institute of Sheep and Goat Breeding. - 2015. – Vol. 1, No. 8. – pp. 424-427.

12. Certificate of state registration of the computer program No. 2025681323 Russian Federation. A program for calculating the pharmacokinetic parameters of exogenous sorbitol in cattle: application 07/01/2025 : published 08/13/2025 / V. S. Ponamarev ; applicant Federal State Budgetary Educational Institution of Higher Education "Saint Petersburg State University of Veterinary Medicine".

13. Gerlachet M., Sendid B., Poulain D., Malaisse W.J. Sensitive and specific determination of sorbitol and fructose in biological samples // Clinical Biochemistry. – 1996. – Vol. 29(3). – P. 295–298.

14. West C. D., Rapoport S. Modification of Colorimetric Method for determination of Mannitol and Sorbitol in Plasma and Urine. Experimental Biology and Medicine. 1949;70(1):141-142.

15. Corcoran A. C., Page I. H. A method for the determination of mannitol in plasma and urine. Journal of Biological Chemistry. 1947;5(1):130.

16. Hokonova T.M., Hokonova M. Z.The role of nonparametric statistical methods in biomedical research // APRIORI. Series: Natural and Technical Sciences. 2014. № 4. URL: https://cyberleninka.ru/article/n/rolneparametricheskih-metodov-statistiki-vmediko-biologicheskih-issledovaniyah (date of request: 09/06/2025).