The use of mesenchymal stem cell lysate as a rehabilitation medium for thawed sperm from breeding bulls

Cryopreservation of gametes is an important component of assisted reproductive technology programs. Considering the properties of mesenchymal stem cells (MSCs) and their derivatives, mainly regeneration (due to cytokines, growth factors, exosomes), the aim of this work was to study the use of MSC lysate as a rehabilitation medium for defrosted sperm of breeding bulls. Mesenchymal stem cell (MSC) lysate is a product of MSC destruction containing their biologically active components: proteins, nucleic acids, exosomes, growth factors and metabolites. After thawing, two groups of bull sperm samples were formed: control and experimental, 15 samples each. Samples of the experimental group were mixed with a medium containing MSC lysate, and those of the control group were mixed with phosphate-buffered saline (PBS) in a 1:1 ratio. Sperm quality parameters (motility, morphology) were assessed after 0, 2 and 4 hours of incubation. Due to the sample size, the statistical hypothesis about the difference between the experiment and the control was tested using the Wilcoxon ttest for paired. It was found that four-hour incubation of spermatozoa after thawing in media containing MSC lysate helps to preserve the greatest number of morphologically normal spermatozoa (71.53±3.21%) with the lowest content of head (8.87±1.10%) and tail (18.33±2.27%) defects. Statistically significant differences (p<0.05) were found in the number of progressively motile and immobile spermatozoa at the 4th hour of incubation. The mean value of progressively motile spermatozoa was 1.4 times higher in the experimental group, and the mean number of immobile spermatozoa in the control group was 1.3 times higher. The obtained data prove the prospects of using the MSC component - lysate as a medium for spermatozoa rehabilitation after thawing. However, further research is needed to precisely determine the mechanism of action.> < 0.05) were found in the number of progressively motile and immobile spermatozoa at the 4th hour of incubation. The mean value of progressively motile spermatozoa was 1.4 times higher in the experimental group, and the mean number of immobile spermatozoa in the control group was 1.3 times higher. The obtained data prove the prospects of using the MSC component - lysate as a medium for spermatozoa rehabilitation after thawing. However, further research is needed to precisely determine the mechanism of action.

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