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International Journal of Veterinary Medicine

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Associative gastrointestinal infection of horses in Leningrad region

https://doi.org/10.17238/issn2072-2419.2020.3.19

Abstract

The article presents the results of a examination of calves under the age of 1 month, kept in one husbandry located on two farms in different districts of the Leningrad region. Diarrhea was found in 45% of calves at the farm in Osminovillage of, Luga district. In the Laboratory for the Study of Parasitic Diseases at the Department of Parasitology named after V. L. Yakimov at the St. Petersburg State University of Veterinary Medicine, the scatological study algorithm consisted of making a direct smear, using the Darling’s method with an improved flotation fluid and staining fecal smears according to Romanovsky-Giemsa’s and Ziehl-Neelsen’s techniques. The staining of smears from feces by the Romanovsky-Giemsa’s and Ziehl-Neelsen’s techniques made it possible to detect pathogens with a size of 3-5 microns, which are typical for Cryptosporidium sp. and Blastocystis sp. For the detection of blastocysts, the preferred diagnostic method is the Romanovsky-Giemsa staining of fecal smears, and for the detection of cryptosporidia - Ziehl-Neelsen staining. Using this algorithm of scatological studies on a livestock farm located in the village of Porozhkino, the parasitic etiology of dysfunction of the gastrointestinal tract in calves was excluded. At the livestock farm in the village of Osmino, a complex of scatological studies was carried out to identify the causative agents of cryptosporidiosis and blastocystosis, and recommendations were given on the implementation of therapeutic and prophylactic measures on this farm. The diagnostic algorithm, which includes a step-by-step study of animal feces by the direct smear method, flotation method, staining of smears according to Romanovsky-Giemsa’s and Ziehl-Neelsen’s techniques allows us to identify a wide range of pathogens and establish not only mono, but also mixed infection.

About the Authors

N. A. Gavrilova
St. Petersburg State University of Veterinary Medicine
Russian Federation


L. M. Belova
St. Petersburg State University of Veterinary Medicine
Russian Federation


Yu. A. Shcherbina
St. Petersburg State University of Veterinary Medicine
Russian Federation


References

1. Белова, Л. М. Мировая фауна и морфофункциональная организация бластоцист/ Л.М. Белова //Зоологический институт РАН - 1992.-54 С.

2. Белова, Л.М. Новая универсальная флотационная жидкость для комплексных лабораторных исследований / Л.М. Белова, Н.А. Гаврилова, Д.Н. Пудовкин // Вопросы нормативно -правового регулирования в ветеринарии. - 2012. - №4/1. - 15-17 С.

3. Кириллов, Е.Г. Криптоспоридиоз: общая характеристика и особенности его распространения/ Е.Г. Кириллов // Ученые записки Казанской государственной академии ветеринарной медицины им. Н.Э. Баумана. - Казань, 2014. - №1. - 128-131 С.

4. Крылов, М. В. Определитель паразитических простейших (человека, домашних животных, сельскохозяйственных растений) / М.В. Крылов, Л.М. Белова// Зоологический институт РАН - 1996. - 608 С. / ил.

5. Кряжев, А.Л. Криптоспоридиоз телят в хозяйствах молочной специализации Северо-Запада России: монография / А.Л.Кряжев,П.А. Лемехов. - Вологда. - Молочное: ИЦ ВГМХА, 2010. - 111с.

6. Никитин, В. Ф. Биолого-эпизоотологические особенности криптоспоридиоза домашних животных и его профилактика / В.Ф. Никитин //Российский паразитологический журнал. -2007. - №. 1. С.36-38.

7. Никитин, В.Ф. Криптоспоридиоз домашних животных / В.Ф. Никитин // М.: ВИГИС, 2007. 34 - 36 с.

8. Успенский, А. В. Современная ситуация по паразитозам и меры борьбы с ними в России и странах СНГ (по материалам Координационных отчетов) / А.В. Успенский, Е.И. Малахова, Т.А. Ершова //Российский паразитологический журнал- М.,2014. - С. 43-50.

9. Che n X. Q. Description of a Blastocystis species from Rattusnorvegicus / Che n X. Q., Sing h ML, Ho L. C., Ta n S. W., Ng G. С., Мое К. Т., Y a p E. H. // Parasitol. Res. 1997. Vol. 83. P. 313-318.

10. Stenze D. J. Morphological differences in Blastocystis cysts - an indication of different species? / Stenze l D. J., Le e M. G., Boreha m P. F. L. // Parasitol. Res. 1997. Vol. 83, N 5. P. 452-457.


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For citations:


Gavrilova N.A., Belova L.M., Shcherbina Yu.A. Associative gastrointestinal infection of horses in Leningrad region. International Journal of Veterinary Medicine. 2020;(3):19-23. (In Russ.) https://doi.org/10.17238/issn2072-2419.2020.3.19

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ISSN 2072-2419 (Print)