Application of NGS for evaluating the symbiotic microflora of the reindeer rumen in the russian Arctic

The method of next generation sequencing (NGS) allows deeply analyze the composition of the microorganisms of the rumen of ruminants. Sampling of the contents of the rumen was carried out in the winter-spring period during the slaughter of reindeer in the territory of the Murmansk region - in the agricultural complex “Tundra” of the Lovozero district. 12 samples of rumen content were taken. Samples of rumen contents intended for molecular genetic studies were frozen immediately after selection at -20 ° C and then placed for long-term storage in a freezer. Total DNA was extracted from the samples using the Genomic DNA Purification Kit (Fermentas Inc., Lithuania), in accordance with the manufacturer's recommendations. The amount of DNA extracted was measured using a Qubit® 2.0 fluorometer (Life Technologies, USA). The extracted total DNA was stored at -20 ° C for further analysis. Evaluation of the bacterial community of the rumen was carried out by NGS sequencing on the next generation sequencing platform MiSeq (Illumina, USA) using primers for 16S rRNA. Sequencing was carried out using reagents for library preparation (Illumina, USA). The processing of the reads (readings of DNA), including overlapping, quality filtration (Q30), and primer trimming, were performed using the Illumina bioinformatics platform. Quality control and data analysis was carried out in accordance with the QIIME2 ver.2019.10 program (https://docs.qiime2.org). NGS samples of rumen content of reindeer in the Murmansk region made it possible to describe the bacterial biodiversity patterns of the microbial community of the reindeer rumen and to reveal a number of pathogenic groups of bacteria. Thus, the use of the NGS method for the analysis of the microbial communities of the reindeer rumen is interesting for identifying various pathogens and characterizing the digestive system and the health of the animal as a whole.

NGS, рубец, Rangifer tarandus, микрофлора, Арктика, NGS, Rumen, Rangifer tarandus, microflora, Arctic

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