Microflora released in mastitis and determination of its sensitivity to antibacterial drugs

A bacteriological study of milk samples from cows with clinical forms of mastitis from four livestock farms of different districts of the Leningrad regione, were conducted. During the bacteriological study of milk from cows with mastitis, we isolated 68 cultures of different types of microorganisms, which indicates the diversity of the microbial “paysage”. Among the isolated microflora, the dominant ones were staphylococci (including hemolytic), microorganisms of the genus Bacillus (including hemolytic), Enterobacteria (Escherichia coli, Klebsiella). Streptococci (including hemolytic), Pseudomonas aeruginosa were isolated. The sensitivity of the isolated cultures of staphylococci to antibacterial drugs was determined by the disco-diffuse method. In order to select effective treatment agents in the dominant species of microorganisms, sensitivity to antibacterial drugs of different groups (aminoglycosides, amphenicols, macrolides, polypeptides, tetracyclines, fluoroquinolones, cephalosporins, and others) was studied. When determining the sensitivity, the best results were shown by antibiotics from the group of aminoglycosides (gentamicin), macrolides (azithromycin), amphenicols (levomycetin), fluoroquinolones (ciprofloxacin, enrofloxacin), cephalosporins (ceftiofur) – more than 50% of the studied cultures were sensitive to them. Lincomycin showed the worst results – all 9 studied cultures of staphylococci were resistant to this drug. When using antibiotics for the purpose of treatment in each individual case, it is necessary to check the sensitivity of crops isolated in the farm to antibacterial drugs. Unsystematic treatment with antibiotics is not only ineffective, but also causes significant damage, as antibiotic resistance develops.

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