Comparative study of the mast cells in the pancreas of young and aged rats
Abstract
Mast cells are able to synthesize many biologically active substances (histamine, prostanoids, neuropeptides, proteases, some cytokines and chemokines) involved in regulation of various processes in organs and tissues in norm and pathology. If damaged, mast cells are activated and secrete the factors they produce in surrounding tissues. The relevance of the study of the pancreatic mast cells is associated with their regulatory function, which is manifested in such socially important diseases of the pancreatic gland as amyloidosis, diabetes mellitus, and pan creatitis. The question of the direct effect of aging on the morphofunctional features of mast cells is still poorly understood. In different organs and tissues, the occurrence of mast cells can either increase or decrease with age. The purpose of this study was a comparative investigation of mast cells in the pancreas of young and aging rats. In Wistar male rats aged 3 months and 18-19 months (n = 8), pancreas was isolated and fixed in a zinc-ethanol-formaldehyde fixative. Paraffin sections 5 pm wide were prepared and stained with toluidine blue to reveal the mast cells. Majority of mast cells were found in the interlobular spaces of the pancreas. They were rarely seen at the acini of lobules. At the locations of the excretory ducts, as well as in the islets of Langerhans, mast cells were practically absent. Single cells were found near the islets, on the border with the exocrine part of the gland. In 3 month-old and aged rats, number of mast cells per square unit were counted in the connective tissue of interlobular space. The density of granular mast cells in the interlobular region of the pancreas of the rat were shown to decrease with aging by approximately 1.5 times. Apparently, the decrease in mast cells is associated with a decrease in the overall immune status of the body during aging.
About the Authors
E. S. Petrova
ФГБНУ «Институт экспериментальной медицины»
Russian Federation
Russian Federation
E. A. Kolos
ФГБНУ «Институт экспериментальной медицины»
Russian Federation
Russian Federation
E. I. Chumasov
ФГБНУ «Институт экспериментальной медицины»; ФГБОУ ВО СПбГАВМ
Russian Federation
Russian Federation
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Review
For citations:
Petrova E.S., Kolos E.A., Chumasov E.I. Comparative study of the mast cells in the pancreas of young and aged rats. International Journal of Veterinary Medicine. 2018;(1):54-59. (In Russ.)
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