Structural and functional changes in blood platelets (trombocytes) when exposed to an electric field (electric field effect)

The purpose of this experiment was to reveal the structural and functional changes in platelets that occur in them under the influence of external factors (electric feld).A galvanic cell was constructed which was a glass capillary with an inner diameter of 0.628 mm length 75 mm (volume = 23.23 mm3) which was filled with the test liquid. the blood of stabilized ЕDТA (ethylenediaminetetraacetic acid). When creating a galvanic cell, the electrode pair was similar to the Leclanche element. A graphite rod and a copper conductor were placed at opposite ends of the capillary. The electrochemical potential was meas ured with a Digital Multimeter Digital DT-832.Before the experiment, blood smears were made. After measuring the potential, blood smears were also made according to a standard procedure, a drop of blood was taken from the side of the graphite rod and the copper conductor. The smears were painted according to Main-Grunwald and Romanovsky-Giemsa Glasses with blood samples were fixed with 1.5% glutaraldehyde solution on phosphate buffer, dehydrated in a series of alcohols of increasing concentration (up to 100%). It was further dried by passing the CO2 critical point, sputtered with gold, and viewed in an electron scanning electron microscope, the Hitachi H-300. In the course of the experiment it was revealed that under the influence of an electric field created by means of a galvanic cell, platelets from cells of normal, diskoid form turned into spherocytes having a number of processes larger than the diameter of the platelets themselves. The altered platelets were assembled into groups of several pieces. Based on registered changes in cells in blood smears, it can be assumed that platelet aggregation occurred due to the action of an electric field in the galvanic cell, which led to platelet fragmentation and increased production of factors involved in blood clotting processes.

тромбоциты, адгезия, агрегация, функции, гемостаз, гальваническая ячейка, platelets, adhesion, aggregation, functions, hemostasis, galvanic cell

1. Кассирский И.А Алексеев Г.А. Клиническая гематология. Изд. 4-е. исп. И. доп.;М. Медицина-1970.

2. Лобовская Л.В. Поверхностная архитектоника и электрофоретическая подвижность интактных и консервированных тромбоцитов. Автореф. дисс. на соиск учен. степ. канд. мед. наук. М.-1985

3. Марковчин А.А. Физиологические особенности тромбоцитов// Научное обозрение. Биологические науки.-2015.-№1.

4. Медведева М. А. Клиническая ветеринарная лабораторная диагностика. Справочник для ветеринарных врачей.-М. «Аквариум Принт», 2013. - 416 с. ил.

5. Ноздрачев А.В. Большой практикум по физиологии человека и животных. В 2 т. Т. 2. Физиология висцеральных систем. учеб. Пособие для студ. вузов/[АД Ноздрачев и др.]; под ред. А.Д. Ноздрачева-М. Издательский центр «Академия», 2007.-544 с.