Morphological changes of the eyeball under the influence of riboflavin in 10-day-old chickens

The effect of a vitamin B2 solution with a concentration of 0.002% and sodium chloride was studied. The research was carried out in the scientific laboratory of the FGBOU in the Velikolukskaya GSHA on the eggs of the meat chicken F15 White Hubbard, purchased at the Lebyazhie Poultry Breeding Factory, Leningrad Region. The incubation was carried out in an incubator IBL-770. The weight of eggs was from 52 to 61 g. Incubation eggs in the amount of 600 pieces were divided into 2 experimental and control groups. In the first experimental group, the eggs were immersed in a solution of vitamin Riboflavin with a concentration of 0.002% by the method of Suleymanova F.I. and Vavilova O.V. In the second experimental group, the eggs were immersed in a 0.9. sodium chloride solution and allowed to stand for 20 minutes. The control group of eggs was not subjected to pre-incubation treatment. During the work, biological control was carried out by means of ovoscoping. The visual analyzer of embryos was examined in the first 10 days of postnatal ontogenesis. Enucleation of the eyes in 3 chickens from each group was performed. The body weight of the embryo and eyes was determined on an HL-400 balance with an error of ± 0.1 mg. Histologically and morphometrically, they were examined in the eyeballs: the shape and its changes, the size of the eye, the retina, the lens and other structural elements. Vitamin B2 has a positive effect, both on the retina of the eye and on the lens as a whole. It is possible to note the period of active growth of layers of the retina and lens fiber on the 10th day of development, as well as a statistically significant difference in their magnitude. In the experimental group, the number of cells per unit area in the ganglionic layer increased by 33.95 ± 1.12 mkm and 32.52 ± 1.04; the outer glial boundary membrane is 18.05 ± 1.67 and 17.55 ± 1.20 mkm, the outer reticular layer is 13.05 ± 1.35 and 12.53 ± 1.52 mkm, the outer nuclear layer is 11.54 ± 1.35 and 12.53 ± 1.52 mkm. On the histological section, under the influence of riboflavin, thickening of almost all layers is noticeable.

зрительный анализатор, морфометрия глазного яблока, рибофлавин, постнатальный онтогенез, visual analyzer, eyeball morphometry, riboflavin, postnatal ontogenesis

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