Vaccine against colibacteriosis of birds and the prevetnive method of colibacillosis of early age chickens

Colibacteriosis persists to stay one of main secondary infection of poultry farms, bringing great economic loses. It is better to take correct preventive measures than to treat birds. There are both inactivated and live vaccines against colibacteriosis. The aim of our work was to create an effective vaccine against this infection, in this article assays dedicated to measurement of protective vaccine concentrations are described. As a result of a series of experiments, a new vaccine against colibacteriosis of chickens, containing an antigen from the strain of E.coli B -5 in a concentration of 1,0 x106-2, 0x106 microbial cells in 1 cm3 of the solvent -physiological solution, was investigated. In one series of experiments, immunization of 22-day chickens per os with a vaccine strain in the volume of 0.5 cm3 with the content of microbial cells 1,0x106, 2,0x106 and 4,0x106 in 1 cm3 of physiological solution per head (5,0x105, 1,0x105 and 2,0x10s microbial cells in 0.5 cm3, respectively) was used. In another series of experiments, immunization of 4-day -old chickens with the same vaccine strains grown at a temperature of 42 °С was performed, this temperature range maximally adapts the vaccine strain to the body of chickens up to 10 days of age. Chickens were vaccinated with dose of 0.5 cm, containing microbial cells 1.0x105 -3x105 in 1 cm3,it is equal to solution 1:20. For the best result of vaccination this concentrations was established experimentally. We can conclude that, the developed vaccine is effective, harmless, has high immunogenic and protective properties due to the formation of antitoxic immunity, does not require special equipment for manufacturing and is easy for administration.

колибактериоз цыплят, живая вакцина, антиген, термолабильный экзотоксин, иммунизация, ферментативная активность сыворотки крови, концентрация микробных клеток, colibacteriosis of chickens, live vaccine, antigen, immunization, pathological changes, microbial concentration

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