Identification of Salmonella Enteritidis and Salmonella Typhimurium by polymerase chain reaction

Outbreaks of salmonellosis continue to occur in the Russian Federation. Each year, approximately 50,000 situations of salmonellosis are usually recorded. Death from this infection may be 0.02-0.04 per 100 thousand inhabitants. Of the salmonellae serovars available in the Russian Federation, S. Enteritidis takes the predominant role - 85%, followed by S. Typhimurium - 12%, S. Infantis - 10% and others - 6%. Mostly S. Enteritidis, S. Typhimurium, S. Infantis, S. Gallinarum-pullorum are most often isolated from birds. The high level of salmonellosis morbidity in poultry farming is associated with a number of factors such as: problems associated with epidemiological study of the causes of salmonellosis, the acquisition of Salmonella resistance to many antimicrobial drugs and, as a consequence, the lack of effective specific prevention. One of the important factors preventing the spread of Salmonella pathogen is the creation of diagnostic methods allowing to quickly detect the disease. The objective of the present study is to develop unique samples of oligonucleotide sequences allowing to detect DNA of microorganisms of the genus Salmonella. Salmonella serovars are difficult to genetically type because of their high degree of identity. Studies conducted in the study of Salmonella pathogenicity conditions have shown that for the identification of Salmonella strains by molecular genetic method can be used as targets of the area of pathogenicity Islands. After analyzing the publications we have selected the gene invA genetic marker -gene spvA for the purpose of receiving a unique sequence of sequences to serovariant S. Turek, S. Enteritidis. Just the sequences of the genes invA and sefA leveled picking up similar at all serovariants Salmonella part, localized in the first half of the gene. With the help of the BLAST program on the NCBI server, the specificity of the selected primers was evaluated. As a result of the search in the database of sequences of GenBank revealed 100% homology of the selected primers with homologous sequences in the genome of the selected genome. The study of samples containing pathological agents of bacterial nature by PCR confirmed the specificity of the selected primers. Positive results were obtained only with samples containing S. Turek, S. Enteritidis. Thus, the selected primers can be proposed for the study of samples of different composition for the detection of the genomes.

сальмонеллез, пт ица, праймеры, диагност ика, молекулярно-генетические исследования, ПЦР, salmonellosis, poultry, primers, diagnostics, molecular genetic studies, PCR

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