Study of general toxicity of pine chlorophyll-carotene paste on Stylonychiamytilus

Abstract

Chlorophyll-carotene paste (CCP) is a product obtained from a gasoline extract of pine woody greens. The basis for the production of CCP are lipids of wood greens. They consist of neutral fats (glycerol esters and higher fatty acids), fatty acids (linoleic, linolenic, etc.), pigments (carotene, carotenoids, chlorophyll), vitamins А, D, E, K, wax, resins, phosphatides, sterols, essential oils, hormones. The physical characteristics of the paste: it’s a thick homogeneous mass of ointments at room temperature, and the paste becomes more diluted at a temperature above 25 ° C; the smell is distinctive coniferous; the color is dark green. The main purpose of our research is to determine the general toxicity of pine chlorophyll -carotene paste. To achieve the goal, we used an express method of determining the general toxicity on the microorganisms Stylonychia mytilus. The method is based on the extraction of various fractions of toxic substances from the sample with acetone and water simultaneously, followed by the impact of these extracts on Stylonychia. The results of experiment evaluate by the survivability of microorganisms in extracts. As a result of completed experiment of determining the general toxicity on the microorganisms Stylonychia mytilus it was identified that the chlorophyll-carotene paste produced by “Tikhvinskiy chimzavod” is nontoxic, since test of aqueous acetone extract and aqueous solution of the sample simultaneously defined by the survival of Stylonychia - 94 % and 84%, respectively. In according with GOST 31674-2012 feed is considered non-toxic if the survivability of Stylonychia in an amount from 80% to 100%.