Mice liver microstructure during different ActiveMix additive injection dosing

A feed additive containing vitamins in the form of a microemulsion, microelements in chelate form has been developed. The principal difference is the absence of propylene glycol in its composition. To eliminate the occurrence of adverse chemical reactions and eliminate the biochemical antagonism of the components, the additive is divided into two mixtures for alternate daily oral administration. The aim of the work is to evaluate the effect on the microscopic organization of the liver of various doses of the components of the additive when administered daily for a month in an experiment on mice. The study was conducted on 92 adult out-bred white mice - males, divided into six experimental and one control group (table 1). Mice received various dosages of premix daily. The premix was administered orally daily, individually, to each animal with alternation of mixtures in accordance with our recommendations. To obtain an amount convenient for administration to mice, the reduced dosages were supplemented with saline to a volume equivalent to other groups. Control animals received a similar amount of saline. The microstructure of the liver was assessed by analysis of histological preparations stained with hematoxylin-eosin on the 7th, 14th and 30th days of the experiment. Introduction to the diet premix ActiveMix does not cause changes orienting-research behavior of animals. In some groups, there are signs of increased motor activity and reduced indirect signs of a stress response. With the introduction of the ActiveMix premix into the diet of mice, a decrease in the severity of fatty liver infiltration of experimental animals is observed. The positive effect of the effect of the premix on the microscopic picture of the liver was noted already on the 7th day of the experiment. The most effective in relation to this effect is the introduction of the additive in dosages of 0.3 and 0.6 g / kg body weight. The use of supplements in doses up to 3 g / kg of body weight does not cause a violation of the microscopic organization of the liver.

печень, гист ология, хелат ы микроэлемент ов, жировая инфильтрация, гепатоциты, liver, histologie, trace elements in chelated form, fatty infiltration, hepatocyte

1. Jackson, H.F., (1982) The effects of parachlorophenylalanine and stimulus intensity on open-field test measures in rats / H.F. Jackson, P.L. Broadhurst // Neuropharmacology. - 1082. - № 21. - P. 1279-1282.

2. Hepatoprotective Effect and Synergism of Bisdemethoycurcumin against MCD Diet-Induced Nonalcoholic Fatty Liver Disease in Mice / S.B. Kim [et al.] // PLoS One. - 2016. - Feb 16;11(2): e0147745. doi: 10.1371/journal.pone.0147745. eCollection 2016.

3. Бабаян, М. Л. Эффективность витаминопрофилактики и выбор витаминноминерального комплекса у детей / М.Л. Бабаян // Вопросы практической педиатрии. - 2011. - Т. 6. - № 4. - С. 85-89

4. Inhibiting triglyceride synthesis improves hepatic steatosis but exacerbates liver damage and fibrosis in obese mice with nonalcoholic steatohepatitis / K. Yamaguchi [et al.] // Hepatology. - 2007. - № 45 (6). - P 1366-1374

5. Pickett-Blakely, O. Micronutrients in Nonalcoholic Fatty Liver Disease Pathogenesis / O. Pickett-Blakely, K. Young, R.M. Carr // Cellular and Molecular Gastroenterology and Hepatology. -2018. - Aug 23;6(4). - P 451-462

6. Sustained hepatic and renal glucose-6-phosphatase expression corrects glycogen storage disease type Ia in mice / M.S. Sun [et al.] // Human Molecular Genetics. -2002. - Sep 1;11(18). - P.55-64