The virulence factors and invasive properties of probiotic strains of microorganisms of the genus Bacillus

Recently probiotic medicines based on microorganisms of the genus Bacillus have been increasingly used in wide veterinary practice. However, despite a large number of publications about positive effect of their use, among practising veterinary experts opinions that these drugs worsen the health of animals, or reduce the quality of the products are heard more often. The article presents data on the results of a study of the presence of factors of pathogenicity and invasive properties of some probiotic strains of microorganisms of the genus Bacillus. The experiments were conducted as in vitro - using standard microbiological techniques set out in the General pharmocopoeia monograph 1.7.2.0012.15 and in vivo - on laboratory animals (white mice of the ISR line, chickens). The presence of the investigated strains of virulence factors, as well as their invasive proper-ties,were estimated. The data obtained in the work indicate that the studied strains represent an environmental and sanitary danger when are used both in veterinary medicine as probiotics and in humanitarian medicine due to the development of a complex of enzymes that are factors of pathogenicity and frank invasive ability, leading to contamination of organs and tissues of the body. Application of these strains is capable to worsen ecological safety of products of poultry farming due to contamination of carcasses of birds with microorganisms of the genus Bacillus and also deteriorations in a trade dress of a bird due to formation of mucous colonies on a carcass surface. According to requirements of general pharmacopoeia monograph 1.7.2.0012.15 -all studied strains cannot be used as probiotic since constitute sanitary danger at application them both in a veterinary medicine, and in humanitarian medicine and are capable to do harm to an organism of an animal and the person.

пробиотики, бациллы, факторы патогенности, инвазивность, контаминация, probiotics, bacillus, pathogenicity factors, invasiveness, contamination

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