Diagnosis of Newcastle disease using the molecular genetic method (PCR)

Newcastle disease is a big threat to poultry. Most often in Kyrgyzstan there are such infectious diseases of birds: Newcastle disease, infectious laryngotracheitis, infectious bronchitis, etc.The reservoir of pathogens can be migratory wild birds, as well as domestic ducks and geese.According to the State Inspectorate for Veterinary and PhytosanitarySecurity under the Government of the Kyrgyz Republic, in August 2012, 4 points were registered for Newcastle disease. Work carried out in the laboratory of Virology and Biotechnology Research Institute named after A. Duysheeva veterinary medicine. As a pathological material, we used lungs of sick birds taken during the outbreak of Newcastle disease in the Kyrgyz Republic in 2012 -2016. The purpose of our work was to identify the virus of Newcastle disease, to conduct serological and molecular biological studies. The investigation of Newcastle disease has been carried out under laboratory conditions, we have to do this, we used chicken embryos to accumulate virus Subsequently, the allantoic liquid was collected from the eggs for the reaction using a molecular biological method of PCR, which showed the following results. Based on the results of PCR analysis, positive results were obtained from 15 samples in these samples of 9, 11, 13, 15. This indicates a pathogenic virus, which confirms the diagnosis of the disease in Newcastle disease. Polymerase chain reaction (PCR) allows detection of fragments of the virus genome regardless of previous vaccination and antibody level. The obtained data will be used for carrying out further research on phylogenetic analysis, to identify and determine the strain of virus.

болезнь Ньюкасла , генот ип , шт аммкуриныеэмбрионы , аллант оис-наяжидкость , РГА , ПЦР , реакционнаясмесь , Newcastle disease , genotype , strain , chicken embryos , allantoic fluid , HA , PCR, reaction mixture

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