Application of gel on the basis of chloroxexidin in treatment of the rane in dogs

Abstract

The object of the study was a wound healing gel containing 4% chlorhexidine, developed at the Department of Pharmacology and Toxicology of St. Petersburg State Medical University. Clinical trials of wound healing gel were carried out on the basis of the veterinary clinic "Nika". For this purpose, two groups of dogs were formed with 10 heads each. The animals in the groups were determined on the basis of analogues, so that the wound size was in the range 3-4 cm. The first, experimental group, after surgical treatment, was treated with 4% gel with chlorhexidine. In the control group, after treatment of the wound surface, treatment was performed with Levomecol ointment. Studies of antimicrobial activity were carried out in vitro by diffusion on solid nutrient media, with reference strains of microorganisms, the main potential causative agents of wound purulent processes: Escherichia coli (strain 259222), Staphylococcus aureus (strain 25923), Pseudomonas aeruginosa (strain 27953), Proteus mirabilis. The account of antimicrobial activity was carried out by measuring the zone of growth retardation of microorganisms. The obtained data allow to draw a conclusion that the purulent inflammation of the wound surface causes the association of conditionally pathogenic microorganisms. An important role in this process is assigned to the coccal microflora, which is 70%. Antibacterial study of 4% gel with chlorhexidine showed high antimicrobial activity against the microflora isolated from the wound surface. Conducted clinical trials suggest that 4% with chlorhexidine gel has a pronounced wound-healing effect. Healing of a wound defect in dogs, in a group where a 4% gel with chlorhexidine was used as a treatment, occurred on day 12, and under the action of Levomecol ointment by day 16.