Research of new properties of Bidens tripartita extract for veterinary practice

It is known that bur-marigold extract (Bidens tripartita) has anti-inflammatory and wound-healing properties and is used to treat diseases animals. The traditional use of this medicinal plant may be due to its unique characteristics to influence the processes of Quorum Sensing (QS) of bacterial communities. The purpose of this study was a comprehensive study of the chemical profile and biological properties of extracts obtained from B. tripartita, for further practical use in veterinary medicine. The study of anti-QS activity in in biosynthesisinhibition tests of violacein was carried out by agar diffusion method, chromatography-mass spectrometry was used to analyze the component composition of the herb. The study of anti-QS activity in biosynthesis inhibition tests of violacein confirmed the increase in pigmentation of the wild C. violaceum strain and the stimulation of the pigmentation of C. violaceum CV026 without the addition of C6-AHL. There was no significant difference in the size and quality of pigmentation, identical results were observed in all cases. Analyzing the results of chromatography-mass spectrometry of active substances of the herb extract, it has been established that most of them are residues of complex sugars, which partially decompose as a result of the preparation of extract and the effect of methanol and nucleic acid derivatives.The chemical structure and known data on biological activity of the identified chemical compounds have been studied; it will help to determine their functioning as possible inhibitors or activators of the QS system. The burmarigold has a unique feature to influence the Quorum sensing system of bacterial communities due to the effect of quorum-dependent pigmentation stimulation, while further study of biological activity of the identified chemical compounds is necessary.

экст ракт , Bidens tripartita, анти-QS активность, ветеринарная медицина, extract, Bidens tripartita, anti-QS activity, veterinary medicine

1. Uysal S., Ugurlu A., Zengin G., Baloglu M.C., Altunoglu Y.C., Mollica A., Custodio L., Neng N.R., Nogueira J.M.F., Mahomoodally M.F. Novel in vitro and in silico insights of the multi-biological activities and chemical composition of Bidens tripartita L. Food Chem Toxicol. 2018 Jan;111:525-536. doi: 10.1016/ j.fct.2017.11.058.

2. Orhan N., Gökgen Ü., Levent І. Altun, and M. Aslan. Anti-hyperglycaemic and antioxidant effects of Bidens tripartita and quantitative analysis on its active principles. Iran J Basic Med Sci. 2016 Oct; 19 (10): 1114-1124.

3. Deryabin D.G., Tolmacheva А.А., Antibacterial and anti-quorum sensing molecular composition derived from Quercus cortex (Oak bark) extract. // Molecules. -2015. - №.9 (20). - Р. 17093-17108

4. Schikora A., Schenk S.T., Hartmann A. Beneficial effects of bacteria-plant communication based on quorum sensing molecules of theN-acyl homoserine lactone group. Plant Mol Biol (2016) 90: 605. https://doi.org/10.1007/s11103-016-0457-8

5. Patel H.K., Suarez-Moreno Z.R., Degrassi G., Subramoni S., Gonzalez J.F., Venturi V. Bacterial LuxR solos have evolved to respond to different molecules including signals from plants. Front Plant Sci - 2013. - V. 4. - P. 447.

6. Лесиовская Е.Е., Пастушенков Л.В. «Фармакотерапия с основами фитотерапии»: Учебное пособие. - 2-е изд. -М.: ГЭОТАР-МЕД, 2003. - 592 с.

7. Abdullah, Asghar A., Butt M.S., Shahid M., Huang Q. Evaluating the antimicrobial potential of green cardamom essential oil focusing on quorum sensing inhibition of Chromobacterium violaceum. J Food Sci Technol. 2017 Jul;54(8):2306-2315. doi: 10.1007/s13197-017-2668-7.

8. Sharifi A, Ahmadi A, Mohammadzadeh A. Streptococcus pneumonia quorum sensing and biofilm formation are affected by Thymus daenensis, Satureja hortensis, and Origanum vulgare essential oils. Acta Microbiol Immunol Hung. 2018 Feb 23:1-15. doi: 10.1556/030.65.2018.013.

9. Bai J., Wu Y., Liu X., Zhong K., Huang Y., Gao H. Antibacterial Activity of Shikimic Acid from Pine Needles of Cedrus deodaraagainst Staphylococcus aureus through Damage to Cell Membrane. Int J Mol Sci. 2015 Nov; 16(11): 27145-27155. doi: 10.3390/ijms161126015.

10. Cicero L. T. Chang, Chih-Yao Chung, Chih-Horng Kuo, Tien-Fen Kuo, Chu-Wen Yang,and Wen-Chin Yang. Beneficial Effect of Bidens pilosa on Body Weight Gain, Food Conversion Ratio, Gut Bacteria and Coccidiosis in Chickens. PLoS One. 2016; 11(1): e0146141. doi: 10.1371/journal.pone.0146141

11. Chang C.L., Yang C.Y., Muthamilselvan T, Yang W.C. Field trial of medicinal plant, Bidens pilosa, against eimeriosis in broilers. Sci Rep. 2016 Apr 21;6:24692. doi: 10.1038/ srep24692.