Study of the antimicrobial activity of the new phytosorption complex

Abstract

The problem of increasing the resistance of microorganisms and a decrease in the effectiveness of antibiotics forces specialists around the world to seek new agents for the treatment and prevention of animal diseases. Essential oils contained in plants have antimicrobial and antioxidant effects. Phytobiotic complexes improve the palatability of the main diet, increase its eating and digestibility of nutrients. The main mechanism of antimicrobial action of components is a decrease in the adhesive ability of microorganisms, the main determining factor of bacterial and fungal virulence. A study on the antimicrobial activity of the new preparation was carried out in vitro by diffusion to agar for the following reference strains of microorganisms-Enterococcus feacalis, Escherichia coli, Proteus mirabilis, Pseudomonas aerugenosa, Salmonella typhimurium, Staphylococcus aureus and Candida albicans. For the study, a slurry containing a standard number of microorganisms was prepared and plated on a layer of nutrient agar in Petri dishes. The seed size was determined according to the procedure described in GF XIII (2016). The account of antimicrobial activity was carried out by measuring the zone of growth inhibition of microorganisms. According to the data obtained, the new phytobiotic complex has a pronounced antimicrobial effect against Escherichia coli and Salmonella typhimurium, 18.2 ± 0.45 mm and 19.2 ± 0.24 mm, respectively. The delay zone for Staphyloccusaureus was 13.0 ± 0.25 mm, for Pseudomonas aeruginosa 10.3 ± 0.21 mm. Thus, the conducted studies showed that the new phytosorption complex has a pronounced antimicrobial effect. This drug may be of interest to veterinary specialists in the treatment of diseases of the gastrointestinal tract and increase the productivity of animals.