Analysis of cellulosolytic microflora of cows by crown method of sequentiation on the genes of 16S rRNA

The research was carried out in TatNIISK FIC KazNC RAS, Kazan (Privolzhsky) Federal University, LLC "Agrofirma Rassvet" Kukmorsky district of the Republic of Tatarstan on milking cows of Kholmogory breed of Tatarstan type. The study continued from the 27th to the 90th day of lactation until the end of the ripening period. The animals were divided into four groups of 20 animals each. Milking cows of the first (control) group received the basic economic diet (OP). Animals of the second, third, fourth (experimental) groups received experimental feed concentrate in addition to OR. This concentrate consisted of a complex of enzymes, probiotic microorganisms, L-carnitine, sapropel, taken in a certain ratio, in the amount of 100, 150 and 200 g per head per day, respectively, which was fed separately as an independent component of the ration in the morning feeding. The composition of the experimental feed additive is developed, and its required amount is produced in Tatar Research Institute of Agriculture of FRC Kazan Scientific Center of RAS. Analysis of the rumen microflora was performed by sequencing the 16S rRNA gene on the IlluminaMiSec platform in Kazan (Volga region) Federal University. Studies have established that the use of cows in the rations of feeding an experimental feed supplement did not have a visible effect on the composition of the rumen microflora as a whole. However, the use of experimental feed additive affected the content of important functional groups of microorganisms of the genera Fibrobacter, Ruminococcus, Anaeroplasma and Ruminobacter. These microorganisms provide carbohydrate metabolism, its intensity. The difference in the Shannon index gives grounds to believe that there is a possible dependence of the productive effect of the test feed additive on the uniformity of the distribution of bacteria in the rumen of the animals themselves. This research was supported by FASO Russia project АААА-А18-118031390148-1

корова, пищеварение, микробиот а, секвенирование, ДНК, метагеном, cow, digestion, microbiota, sequencing, DNA, metagenome

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