Effective methods for detecting respiratory pathogens in the biological material of birds

Abstract

Bacterial diseases with an aerogenic path of spread remain the main difficult problem for many poultry farms. Modern rates of technological development in poultry farming, in turn, can lead to various disorders on the part of the bird's body. In addition, under the influence of external factors and the macro organism itself, changes in pathogenic and opportunistic microorganisms occur. In particular, the acquisition of their resistance to the action of drugs, changes in their virulent properties lead to the fact that in the body of birds they show a course of disease that is not characteristic of them, sometimes cause an atypical course. Together, all these factors make it difficult to make a diagnosis, which in turn leads to incorrect or not timely treatment.

The aim of our research was to determine the spectrum of pathogens circulating in poultry farms that cause respiratory diseases of birds. We have identified the most common pathogens of these diseases, and their isolation from various biological materials was of particular interest. Samples of samples from different poultry farms from clinically ill birds, fallen birds, as well as birds that do not have clinical manifestations of the disease were examined in case of detection of bacteriocarriage. The studies were conducted using standard methods used in bacteriology, as well as molecular genetic diagnostic methods. This approach to the use of different diagnostic methods allows us to determine the most effective, fast and accurate methods for identifying bacterial pathogens of respiratory diseases in birds.