Efficacy of a complex medicinal herbal drug in the modeling of oxalate nephrolithiasis in rats

Abstract

In this study, a comparative analysis of the therapeutic effect of a complex medicinal herbal drug based on extracts from thinleaved killer whale and “Cystone”TM in combination with drotaverine hydrochloride in oxalate nephrolithiasis in rats was carried out. For 45 days, the animals were fed 0.75% ethylene glycol with a 2% solution of ammonium chloride using a gastric tube. 45 days after the administration of these drugs, to confirm the development of urolithiasis in some animals, blood samples (from the posterior vena cava) and urine (metabolic cells, 12 hours) were taken for biochemical analysis with the determination of the following parameters: urea, creatine, albumin, alkaline phosphatase, AlT, AsT, ALP, CHO, BUN, CRE, GGT (automatic analyzer XL Erba Lachemab), general urine analysis with the determination of leukocytes, ketones, nitrites, urobilinogen, bilirubin, protein, glucose, density, blood, pH (on the CL-50 – HT urinary analyzer). Ultrasound examination of the kidneys of rats on the model of oxalate nephrolithiasis did not reveal visible conglomerates, which did not allow this method to diagnose the development of ICD in rats. The most positive pharmacodynamic effect on urodynamics and urine parameters was demonstrated by the drugs used in a complex for the 2nd group of animals-dietary supplements “Nephromon” + drotaverine, probably due to their rapid nephroprotective and antispasmodic effects. The combined use of the drugs “No-shpa”TM (drotaverine hydrochloride) and “Cystone”TM, dietary supplement “Nephromon-Plus” in two different groups of laboratory rats showed a therapeutic effect. Against the background of the therapy, the animals normalized the clinical parameters of nephrolithiasis: blood and urine tests.