Histological features of the liver of newborn landrace piglets

Abstract

The study was conducted at the Department of animal anatomy of the Saint Petersburg state University of veterinary medicine. Cadaver material for the study was delivered from the Idavang agro pig breeding complex in Nurma, Tosnensky district of the Leningrad region. The objects for this study were newborn piglets at the age of one day. A sample of piglets ' liver was subjected to histological examination. The material was fixed in a 10% solution of neutral formalin for 24 hours, after which it was poured into paraffin according to the generally accepted method. Then sections 5-7 microns thick were made, which were stained with hematoxylin and eosin and Masson trichrome in order to detect collagen fibers. Some sections were stained with Alcian blue (pH 2.5) followed by Mayer's hematoxylin and tolluidine blue. Histological preparations were analyzed using a Carl Zeiss Axio Skop 2 plus light-optical microscope (Germany) at magnifications of 40, 100, 200, and 400. The study established histological features of the liver structure of newborn piglets at the age of one day. Unlike sexually mature individuals, this organ at the age of one day has its own characteristic histological features, in particular, an ordered beam structure consisting of large hepatic lobes formed by tightly arranged strands of hepatocytes. Hepatocytes, which form the bulk of the organ parenchyma, are oval and polygonal in shape, with well-defined cell boundaries, abundant fine-grained eosinophilic, sometimes finely vacuolated, almost transparent cytoplasm, and one, less often two centrally located rounded nuclei with evenly distributed, small - and large-lobed chromatin.