The influence of the morphofunctional state of the ovaries on the efficiency of obtaining embryos in vitro
Abstract
It is impossible to solve the problem of modernization and dynamic development of dairy cattle breeding without qualitative improvement and renewal of the existing livestock. The research was carried out within the framework of two state research programs: "Biotechnology" (sub-program "Development of biological science, biological education and biological industry for 2007-2011 and for the period up to 2020"), "High-tech technologies and equipment for 2016-2020" (sub-program 1 "Innovative Biotechnologies-2020"). The research was conducted on the basis of the branch biotechnological laboratory for reproduction of farm animals of the State Educational Institution "Grodno State Agrarian University". This paper presents the results of studies on the influence of the morphofunctional state of the ovaries on the yield of preimplantation stage embryos in in vitro culture. According to the results of the studies, the morphological parameters of the ovaries were on average: length -33.1±4.62 (lim – 21.3-43.1 mm), width-19.2±1.32 (lim – 10.9-27.1 mm) with a coefficient of variation of 26.6%. The ovarian volume averaged 6.8±0.62 (lim – 3.5 -12.1 cm3). There is a tendency to increase the number of follicles and oocytes in ovaries with a volume of more than 6.0 cm3, while the difference in the number of follicles with a diameter of 2-4 mm is significant (P<0.05). More effective was the use of ovaries with more than 20 follicles and a diameter of 3 to 8 mm. The yield of viable embryos in this case was 20.7-23.2% of the number of fertilized oocytes and significantly exceeded the same indicator for other ovarian groups at P><0.01. The obtained data are of practical significance for the development of technology for obtaining embryos in vitro, the use of which will help to accelerate the selection process and increase the efficiency of breeding work in cattle breeding in general.>˂ 0.05). More effective was the use of ovaries with more than 20 follicles and a diameter of 3 to 8 mm. The yield of viable embryos in this case was 20.7-23.2% of the number of fertilized oocytes and significantly exceeded the same indicator for other ovarian groups at P˂ 0.01.
The obtained data are of practical significance for the development of technology for obtaining embryos in vitro, the use of which will help to accelerate the selection process and increase the efficiency of breeding work in cattle breeding in general.
About the Author
L. V. Golubets
"Grodno State Agrarian University"
Russian Federation
Russian Federation
Doctor of Agricultural Sciences Sciences, Ch. scientific. sotr. branch biotechnology lab. on reproduction with farm animals,
Grodno
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Review
For citations:
Golubets L.V. The influence of the morphofunctional state of the ovaries on the efficiency of obtaining embryos in vitro. International Journal of Veterinary Medicine. 2021;(1):333-338. (In Russ.)
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