Experimental obtaining of t-2 toxin and their metabolites from field isolates of fusarium fungi

 The widespread occurrence of filamentous fungi of the genus Fusarium is a very dangerous mold micromycete. Mold isolate of the genus Fusarium can manifest itself in all climatic areas. The micellar fungus is capable of infecting the vascular system of plants, germinated grains, tree roots, and vegetable crops. When tissues are damaged by fungi of the genus Fusarium, plants, as a rule, wilt and begin to rot. Spores and particles of mycelium of the fungus can be localized for  a long time on the surface of the soil, in water, in plant residues, affecting
immature seedlings of grain and agricultural crops. High humidity, lack of daylight hours and temperature drops have a beneficial effect on the toxin  formation of fungi of the genus Fusarium. As an experiment, soil isolates of fungi of the genus Fusarium (Fusarium sporotrichioides) showing the toxin formation of T-2 toxin were selected.
The prepared grain substrate was contaminated with field isolate and exposed  to temperature fluctuations, artificially creating stress for the micromycete. The weediness, growth, and color of mold on different types of grain crops were visually recorded. In addition, suspensions with lactose, beckon, and dimethyl sulfacid were added to the extract to improve growth and toxin formation.
The aim of this work was to study an isolate of the genus Fusarium (Fusarium sporotrichioides) in obtaining T-2 toxin, use for laboratory purposes as a test object and to replenish the collection of microorganisms. 

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