Identification of salmon caviar using PCR-RV

Abstract

To date, there are many ways to detect the falsification of caviar. These include organoleptic and laboratory research methods. However, when adding counterfeit in the amount of up to 25% of the total weight of the product, it is quite difficult to establish the fact of falsification. That is why in veterinary and sanitary practice, polymerase chain reaction is increasingly resorted to. The purpose of the research was to identify the species of salmon fish eggs using polymerase chain reaction with real-time observation (PCR-RV). Identification was carried out in two stages. At the first stage, the eggs were homogenized with subsequent DNA extraction by the adsorption method using a set of "Sorb-GMO-A" (Syntol, Russia). At the second stage, PCR-RV was performed on the Rotor-Gene Q amplifier (Qiagen, Germany) using the Pink Salmon/Chum Salmon/ Sockeye Salmon (Syntol, Russia) and Salmon/Trout test systems/Kizuch" (Organic Test, Russia). The results were evaluated by the kinetic growth curve of the fluorescence signal depending on the DNA present in the studied caviar and the threshold cycle value Ct ≤. 35.

The existing organoleptic and physicochemical methods of veterinary and sanitary examination of caviar make it possible to determine its quality and safety. It is extremely difficult to determine the species of caviar using these methods. Polymerase chain reaction is the most sensitive method for identifying the species of caviar and revealing the facts of its falsification.