The effect of the blood sampling method on the risk of mechanical hemolysis of erythrocytes in healthy calves and with endogenous intoxication syndrome
https://doi.org/10.52419/issn2072-2419.2022.1.110
Abstract
In the conditions of the milk production complex in the Voronezh region, where Holstein cattle are kept, studies were conducted to study the degree of injury to blood cells during its selection by various methods in healthy calves and with endogenous intoxication syndrome. In healthy animals, blood sampling by passive leakage does not affect the membranes of erythrocytes. When using vacuum systems, there are no visual signs (hemolysis) of cell destruction, but changes in their cytoskeleton occur with an increase in the sensitivity of membranes to negative pre-analytical factors. In animals with endogenous intoxication syndrome, there are explicit or hidden destruction of erythrocyte membranes, which are manifested by an increase in the content of extra-erythrocyte hemoglobin, the degree of physiological hemolysis, sensitivity to intra- and extravascular hemolytic factors. As a result, already when selecting blood samples by passive gravity, there is a risk of hemolysis, and when using a vacuum system, it is observed in most samples. Therefore, when examining sick animals, it is necessary to take into account the high probability of laboratory diagnostic error at the pre-analytical stage that occurs due to hemolysis of erythrocytes with hypersensitivity to extravascular cytolytic factors, including mechanical effects during blood sampling.
About the Authors
Yu. N. Alekhin
All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy
Russian Federation
Russian Federation
doctor of veterinary sciences, chief researcher
M. S. Zhukov
All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy
Russian Federation
Russian Federation
candidate of veterinary sciences, senior researcher
G. V. Nikonenko
All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy
Russian Federation
Russian Federation
junior research
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Review
For citations:
Alekhin Yu.N., Zhukov M.S., Nikonenko G.V. The effect of the blood sampling method on the risk of mechanical hemolysis of erythrocytes in healthy calves and with endogenous intoxication syndrome. International Journal of Veterinary Medicine. 2022;(1):110-116. (In Russ.) https://doi.org/10.52419/issn2072-2419.2022.1.110
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