Evaluation of the quality of native and cryopreserved semen of birds of breeds brama light and brama fawn and their chimeras
https://doi.org/10.52419/issn2072-2419.2022.1.175
Abstract
Due to the significant decline in the diversity of bird breeds and populations, there is a need to preserve germ cells of rare breeds and unique organisms, such as chimeras. The main method for preserving the gene pool in birds is the method of semen cryopreservation, which is used in many countries of the world in the form of creating cryobanks. Sperm from the birds of the breeds Brama light and Brama fawn and their chimeras were obtained by abdominal massage. Cryopreservation of the semen was carried out in small granules with the addition of a 6% solution of dimethylacetamide. The granules were thawed on a heated metal plate at 60°C. The ejaculate volume, sperm activity and its concentration in the studied breeds and their chimeras did not differ statistically. The maximum activity of the frozen-thawed semen was observed in the semen of the Brahma fawn breed (40.0%), and the minimum activity in the Brahma light breed was 30%, while in chimeras the semen activity occupied an intermediate position and amounted to 35%. Thus, frozen-thawed spermatozoa of bird chimeras are viable, as well as native semen.
Keywords
About the Authors
L. V. Kozikova
Russian research institute of farm animal genetics and breeding - branch of the L.K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
Dr. habil.(Biol. Sci), a leading researcher at the Laboratory of Molecular Genetics
E. Polteva
Russian research institute of farm animal genetics and breeding - branch of the L.K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
Junior researcher at the Laboratory of Molecular Genetics
A. A. Kurochkin
Russian research institute of farm animal genetics and breeding - branch of the L.K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
Junior researcher at the Department of genetics, breeding and conservation poultry's genetic resources
N. V. Pleshanov
Russian research institute of farm animal genetics and breeding - branch of the L.K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
Researcher at the Department of genetics, breeding and conservation poultry's genetic resources
References
1. Фисинин В. И. Криоконсервация мужских половых клеток как метод сохранения генетических ресурсов сельскохозяйственной птицы / В. И. Фисинин, В. А. Багиров, Н. А. Волкова, Н. А. Зиновьева, Я. С. Ройтер / / Достижения науки и техники АПК. — 2012. — №8.
2. Purdy P.H. Evaluation of glycerol removal techniques, cryoprotectants, and insemination methods for cryopreserving rooster sperm with implications of regeneration of breed or line or both / P. H. Purdy, Y. Song, F. G. Silversides and H. D. Blackburn / / Poultry Sci. — 2009. — V. 88. — P. 2184-2191.
3. Тагиров М.Т., Замораживание клеток ранних эмбрионов кур. Проблемы криобиологии, тезисы докладов Всеукраинской научной конференции. / Тагиров М.Т., Артеменко А.Б., Кальченко Ю.В. // Успехи и перспективы развития криобиологии и криомедицины", - 2001, - N 3. - С.56.
4. Nicolaia Iaffaldano Italian semen cryobank of autochthonous chicken and turkey breeds: a tool for preserving genetic biodiversity. / Nicolaia Iaffaldano, Michele Di Iorio, Icon, Giusy Rusco, Emanuele Antenucci, Luisa Zanibonib //Journal of Animal Science. V. 20, 2021 - Issue 1. Р. 2022-2033
5. Козикова Л.В. Создание фенотипических химер кур с использованием бластодермальных клеток пород брама светлая и брама палевая. / Л.В. Козикова, Е.А. Полтева // Птицеводство – 2021 - №6. – С.7- 11
6. Целютин К. В. Криоконсервация спермы птиц — как инструмент сохранения генофонда / К. В. Целютин, Б. К. Тур / / Генетика и разведение животных — 2009. — №1. – С. 50-52.
7. Наук В. А. Усовершенствованный метод длительного сохранения семени быков производителей в глубокозамороженном состоянии / В. А. Наук / / Совершенствование методов воспроизводства сельскохозяйственных животных. — Кишинев, 1979. — С.3-37
8. Tajima, A., M. Production of germ-line chimeras by transfer of cryopreserved gonadal primordial germ cells (gpgcs) in chicken. /J Tajima, A., M. Naito, Y. Yasuda, and T. Kuwana.. ||Exp. Zool.-1998-V. 280-P.265–267.
9. Naito M., Tajima A., Tagami T., Yasuda Y., Kuwana T. 1994 Preservation of chick primordial germ cells in liquid nitrogen and subsequent production of viable offspring. Naito M., Tajima A., Tagami T., Yasuda Y., Kuwana T. |Journal of Reproduction and Fertility-1994-V. 102,-P. 321-325.
10. Грищенко В. И. Проблемы криобиологии и сохранение генетических ресурсов /В. И. Грищенко, Е. Ф. Копейка, М. П. Петрушко // Материалы международной конференции «Сохранение генетических ресурсов». Цитология.- 2004.- Том 46.- № 9.- С. 784.
Review
For citations:
Kozikova L.V., Polteva E., Kurochkin A.A., Pleshanov N.V. Evaluation of the quality of native and cryopreserved semen of birds of breeds brama light and brama fawn and their chimeras. International Journal of Veterinary Medicine. 2022;(1):175-180. (In Russ.) https://doi.org/10.52419/issn2072-2419.2022.1.175
Views:
305
Email this article 