Variety range of circulating salmonella serotypes in poultry farms

Abstract

Salmonellosis, as zooantroponoze infection, continues to be one of the actual problem of modern poultry. Among the Salmonella serotypes isolated in Russia, Salmonella enteritidis occupies a dominant position, followed by Salmonella typhimurium and Salmonella infantis. A characteristic feature of this disease in birds is that the infected individuals continue to release the pathogen into the environment and thus pose a serious danger to consumers, since the human body is extremely susceptible to Salmonella. The main objective of our research was to study the variety range of circulating Salmonella serovariants in poultry farms. Samples for assay were received from various biological material from alive and dead birds. A total of 200 different samples from 7 poultry farms were examined. Detection and genotyping of causative agents of avian salmonellosis was carried out using molecular biological method - polymerase chain reaction. Polymerase chain reaction (PCR) has several advantages because it combines speed and ease of execution, as well as potentially high specificity and sensitivity. For this reaction, specific primers developed by us earlier were used. As a result of the research, it was found presence of a specific DNA fragment with a length of 500 BP, that indicates the presence in the studied material of S. enteritidis DNA, and a DNA fragment with a length of 250 BP for S. typhimurium DNA. The presence of serovariant S. infantis in the DNA sample is confirmed by the detection of a fragment length of 324 Bp. The results of the study showed that serovariant S. enteritidis were detected in 54 samples, S. typhimurium 33 samples, and serovariant S. infantis in 32 samples.