Molecular genetic identification of Helycobactersuis in pigs

Abstract

Ulcerative lesions of the gastric mucosa in pigs are recorded regardless of age, gender and breed in all industrial pig breeding systems. It is believed that the most common cause of peptic ulcer in pigs is feed factors associated with the physical qualities of the feed, technological stress, metabolic disorders, and poisoning. Peptic ulcer is a disease of the stomach of pigs of different age groups, of different sex and breed, it is widely spread among piglets, especially during the growing and fattening period (3-6 months). Etiological and pathogeneticfactors for stomach ulcer in pigs remain unclear. The aim of this work was to study the spread of Helicobacter pylori in pigs by laboratory and molecular genetic methods in the pig breeding complex of Kamsky Bacon LLC of the Republic of Tatarstan, as well as to study the possible relationship between the presence of bacteria and the presence of pathology of the gastrointestinal tract of pigs. We used macroscopic, microscopic, cultural, biochemical and molecular genetic methods to study the prevalence and special features of Helikobacter infection in the stomach mucosa of pigs. As a result of the assessment of the macroscopic changes in the stomach mucosa of pigs it was found that there are patho-anatomical changes in 62% of the studied cases, characterized by the presence of hyperkeratosis and erosions. Bacteria of the genus Helicobacterspp. ,was isolated from the stomach mucosa samples by laboratory methods. Molecular-genetic method of revealedH. suis DNA, and the samples were negative for H. pylori DNA genes