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ALUE OF PATHOMORPHOLOGICAL STUDIES FOR DIAGNOSTIC OF INTESTINAL PARASITOSES IN BIRDS

https://doi.org/10.17238/issn2072-2419.2019.4.25

Abstract

The study of the corpses of chickens of Czech Golden breed, died at the age of 7 months, belonged to a private farm located in the Leningrad Region was carried out in a prosectorium of the Department of Pathological Anatomy and Forensic, St. Petersburg State Academy of Veterinary Medicine using a method of incomplete helminthological autopsy according to K. I. Skryabin. We found the presence of nodules on the mucous membrane of the esophagus, its hyperemia, erosion (in some places), thickening and protrusion of the walls of the esophagus, and hyperemia and swelling of the mucous membrane of the small and large intestines, the presence of a large volume of mucus in their contents. This method allowed us to identify only the largest helminths (Ascaridia galli), but did not give us a complete picture of the parasitofauna of dead birds. An additional parasitological study of cadaver material was carried out in the laboratory for the study of parasitic diseases at the Department of Parasitology. For coprological studies, 5 g of the content of the large intestine of the dead bird were taken and examined using the Darling’s method with an improved composition of flotation liquid. Scrapes from the mucous membrane of the small and large intestines, taken in areas of its hyperemia, edema, impaired integrity, were placed on fat-free glass slides, fixed with ethanol and stained according to Romanovsky-Giemsa. Eimeria maxima oocysts, helminth eggs of A. galli and Capilaria sp. (Thominx sp.) were found due to the flotation method. Microscopy of smears revealed the endogenous stages of the development of eimeria - meronts and merozoites. Using the diagnostic method, used mainly for intravital confirmation of the diagnosis, it was possible to detect a number of pathogens that were not detected visually during the autopsy. Post-mortem diagnosis, including the traditional method of incomplete helminthological autopsy according to K. I. Scryabin and coprological methods, in particular Darling’s one with an improved flotation liquid, gives a more complete picture of the parasitofauna of the died organism, which is necessary for making an accurate diagnosis.

About the Authors

N. A. Gavrilova
St. Petersburg State Academy of Veterinary Medicine
Russian Federation


L. M. Belova
St. Petersburg State Academy of Veterinary Medicine
Russian Federation


A. A. Kudryashov
St. Petersburg State Academy of Veterinary Medicine
Russian Federation


O. A. Loginova
St. Petersburg State Academy of Veterinary Medicine
Russian Federation


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Review

For citations:


Gavrilova N.A., Belova L.M., Kudryashov A.A., Loginova O.A. ALUE OF PATHOMORPHOLOGICAL STUDIES FOR DIAGNOSTIC OF INTESTINAL PARASITOSES IN BIRDS. International Journal of Veterinary Medicine. 2019;(4):25-30. (In Russ.) https://doi.org/10.17238/issn2072-2419.2019.4.25

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ISSN 2072-2419 (Print)