Development of the unified intestinal medium for microbiological research in veterinary medicine

Artificial intestinal media are in high demand in microbiological research in the field of veterinary medicine, since they allow obtaining a qualitative and quantitative composition of microbiota corresponding to the natural conditions. The only artificial intestinal medium currently available in domestic microbiological practice has not been validated and its use is limited to the cultivation of lactobacilli. In this regard, the development of a unified artificial intestinal medium (UAIM) that best matches the natural conditions of the intestine, using the example of Brown Nick chickens, is of scientific interest for the effective cultivation of microorganisms during microbiological research in veterinary medicine. In the course of the studies, existing artificial intestinal media were analyzed and key components were selected, including pectin, xylan, arabinogalactan, amylopectin, casein, starch, bactopeptone, salts and vitamins, which ensures the reproduction of natural intestinal conditions. To test the effectiveness of the medium, ten samples of chicken feces were selected, five of which were incubated under UAIM conditions, and the other five were cultured without the use of the medium. The resulting bacterial cultures were identified and quantitatively analyzed using MALDI-TOF mass spectrometry and the method of seeding on selective media. Statistical analysis showed no significant differences in the microbiota composition between the experimental groups (p>0.05), indicating that the intestinal conditions were successfully reproduced using UAIM. This study demonstrates the potential of UAIM for culturing intestinal microbiota and can serve as a basis for further studies aimed at modeling intestinal conditions in the development of veterinary drugs and feeds.

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