IDENTIFICATION OF ORGANOPHOSPHORUS COMPOUNDS BY THIN LAYER CHROMATOGRAPHY

Abstract

In conditions of intensive chemicalization of agriculture, cases of contamination of agricultural products with residual amounts of pesticides and veterinary drugs are increasing. A significant role among them is played by organophosphorus compounds, which, despite the relatively low danger to the body, are capable of accumulating in the fatty tissue of animals, which poses a threat to human health. Due to the wide variety of chemical groups of the substances used, differences in their physicochemical properties, method of application and environmental conditions, it is often difficult to accurately identify contaminants. Organoleptic, physicochemical indicators, as well as mobility coefficients of residual amounts of diazinon and chlorophos were determined by TLC, using UFK 254/365 - HD. At the same time, there was no change in organoleptic and physicochemical indicators in the test samples, which does not allow us to establish contamination of the products. During chromatography, residual amounts of organophosphorus compounds were detected, mobility coefficients Rf of diazinon were determined to be 0.65±0.01 and chlorophos to be 0.19±0.01, which corresponds to the value of control solutions. The use of available components and domestic equipment, as well as the possibility of parallel determination of a large number of samples, allows this method to be used as a screening method to ensure food security.