The effectiveness of the use of mesenchymal stem cells after cryopreservation of sperm from producing goats

The aim of the study was to establish the efficiency of using mesenchymal stem cells from adipose tissue and bone marrow of goats after sperm cryopreservation. The results of four-hour incubation of sperm samples after thawing indicate a reliable difference in the values during incubation with mesenchymal stem cells (MSCs) from adipose tissue/bone marrow and without them (OptiXcell medium). Thus, during four -hour incubation of sperm after thawing, a statistically significant decrease in the number of normal spermatozoa was noted in all study groups. At the same time, the difference in values in the control group was 1.3 (p<0.01, 2 hours) and 1.4 (p<0.01, 4 hours) times; the first experimental group - 1.1 (p<0.01, 4 hours) times; the second experimental group – 1.1 (p < 0.01, 2 hours) and 1.3 (p < 0.01, 4 hours) times compared to the results of 0 hours of incubation. Samples of the control and first experimental groups had the same dynamics of reliable changes in sperm motility during four-hour incubation after thawing – a statistically significant decrease in the number of progressively moving sperm by 1.6 times (p < 0.05 – control group, p < 0.01 – first experimental group), and, on the contrary, a reliable increase in the number of non-progressively moving sperm by 1.3 times (p < 0.05 for both groups). Pronounced motility activity was observed in the sperm of the first experimental group upon completion of 4-hour incubation, equal to 21.45 ± 4.58%. The highest number of viable spermatozoa was registered in the first experimental group with 4-hour incubation of samples with MSC obtained from bone marrow - 37.13±2.21%, the highest number of spermatozoa with intact acrosomes - in the second experimental group (MSC AT). Thus, it can be assumed that MSC has a regenerative effect on the structure of spermatozoa after cryopreservation. To reveal the mechanism of the effect of MSC on the structure of spermatozoa, it is necessary to continue research.

1. Bazhenova N. B., Plemyashov K. V., Korochkina E. A. Otsenkakachestvennykhpokazateleyspermyzhivotnykh. SanktPeterburg:SanktPeterburgskiygosudarstvennyyuniversitetveterinarnoymeditsiny, 2023. 25 s.

2. GOST 32222–2013.Sredstva vosproizvodstva. Sperma. Metodyotbora prob. – M.: Standart inform. 2018. 10 s.

3. Korochkina, E. A. Influence of mesenchymal stromal cells on the quality of ram sperm after cryopreservation / E. A. Korochkina, V. S. Pushkina // Veterinary science and feeding. - 2024. - No. 4. - P. 55-59. - DOI 10.30917 / ATT-VK-1814-9588-2024-4-10.

4. Miroshina, T. A. Prospects for Dairy Goat Breeding / T. A. Miroshina // Problems of Intensive Development of Animal Husbandry and Their Solution: Proceedings of the International Scientific and Practical Conference, Bryansk, March 25–26, 2021. – Bryansk: Bryansk State Agrarian University, 2021. – Pp. 529–532.

5. Toshchev, V. K. Exterior Features of White Russian Goats and Their Crosses with Saanen Goats / V. K. Toshchev, E. V. Tsaregorodtseva, G. N. Mustafina // Mosolov Readings. – Yoshkar-Ola, 2006. – Issue 8. – Pp. 247–249.

6. Goat Semen Cryopreservation Protocol. // National Animal GermplasmProgram. - 2016. (5)

7. Xu, B. Evaluation of lipidomic change in goat sperm after cryopreservation / Xu B, Wang R, Wang Z, et al. // Frontiers in veterinary science. - 2022.-Vol. 9.- P 1-11. (9)

8. Zhang, W. Beneficial effect of proline supplementation on goat spermatozoa quality during cryopreservation / Zhang Weijing, Lingjiang Min, Yajing Li, et al.// Animals. - 2022. - Vol. 12(19). -P. 1-13.(10)