Identification and probiotic properties of lactobacilli from the intestines of broiler chickens
O. V. Zvereva,
Yu. P. Kuznetsova,
A. S. Vostrikova,
M. Yu. Syromyatnikov,
E. V. Mikhaylov,
O. S. Korneeva https://doi.org/10.52419/issn2072-2419.2025.3.152
Abstract
The objects of study in this work were the following strains: Pediococcus acidilactici, Lactobacillus johnsonii and Ligilactobacillus salivarius, isolated from the feces of 14-day-old broiler chickens. A sample of broiler chicken feces was placed in a sterile MRS nutrient medium (HiMedia, India) and incubated at 37 ˚C for 24 hours. After that, the analyzed samples were subcultured deeply onto the MRS-agar nutrient medium (SPC Biokompas-S, Russia) using the limiting dilution method to isolate individual colonies of microorganisms. Petri dishes with the cultures were incubated for 48 hours at 37 ˚C. The grown colonies of microorganisms were identified by cultural and morphological characteristics for further sequencing. The resistance of these cultures to bile acids was studied, their antagonistic activity, tolerance to extremely low and high pH values were assessed, and their resistance to different groups of antibiotics was analyzed. L. salivarius was the most resistant to bile compared to L. johnsonii and P. acidilactici. P. acidilactici did not exhibit antagonistic action against the normal microbiota of the gastrointestinal tract, unlike the other analyzed strains, but had a significant effect on opportunistic bacteria. Extremely low and high pH values did not provoke complete death of P. acidilactici, while L. salivarius and L. johnsonii did not retain activity at extremely low pH values. The experiment also revealed that L. johnsonii was the most antibiotic-sensitive strain.
Supporting agencies: The research was carried out within the state assignment of Ministry of Science and Higher Education of the Russian Federation (project FZGW-2024-0003).
About the Authors
O. V. Zvereva
Voronezh State University of Engineering Technology
Russian Federation
Russian Federation
Assistant Professor of the Department of Biochemistry and Biotechnology
Yu. P. Kuznetsova
Voronezh State University of Engineering Technology
Russian Federation
Russian Federation
Junior Researcher
A. S. Vostrikova
Voronezh State University of Engineering Technology
Russian Federation
Russian Federation
Junior Researcher
M. Yu. Syromyatnikov
Voronezh State University of Engineering Technology;
All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy
Russian Federation
Russian Federation
PhD in Biological Sciences, Associate Professor, Leading Researcher
E. V. Mikhaylov
All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy
Russian Federation
Russian Federation
PhD in Veterinary Sciences, Leading Researcher
O. S. Korneeva
Voronezh State University of Engineering Technology
Russian Federation
Russian Federation
PhD in Biological Sciences, Professor
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Review
For citations:
Zvereva O.V., Kuznetsova Yu.P., Vostrikova A.S., Syromyatnikov M.Yu., Mikhaylov E.V., Korneeva O.S. Identification and probiotic properties of lactobacilli from the intestines of broiler chickens. International Journal of Veterinary Medicine. 2025;(3):152-163. (In Russ.) https://doi.org/10.52419/issn2072-2419.2025.3.152
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