Prospects of using a biocomposition based on Bacillus subtilis bacteria as a safe additive and preventive agent-neutralizer of zearalenone in poultry farming

This document emphasizes the development of feed additives for animals, veterinary drugs, and mineral supplements, supported by the implementation of competitive domestic technologies based on the latest scientific achievements. The goal of this study was to explore the potential use of a biocomposite made from montmorillonite clays and the bacterial strain Bacillus subtilis VKM B-3701D in poultry farming. This biocomposite was tested as a zearalenone mycotoxin neutralizer, as well as a prophylactic and probiotic agent. Zearalenone is a secondary metabolite produced by toxigenic mold fungi, commonly found in contaminated animal feeds, including grains. Even moderate or low levels of zearalenone consumption often led to reduced productivity, such as slower growth rates, decreased production, and lower market quality. These effects frequently occur without clear symptoms of primary mycotoxicosis. The study showed that the biocomposite, when prepared in a 5:1 ratio of bacterial biomass to montmorillonite, reduced the concentration of zearalenone mycotoxin by 6,5 times in vitro experiments, as determined by highperformance liquid chromatography (HPLC). Adding the experimental feed additive to the diet of laying hens at a dosage of 2.0 kg per ton of dry feed increased the average daily weight gain by 5.8% compared to the control group. The average weight per chick reached 3016.9 grams, which is 5.7% higher than the control group. No significant changes in biochemical parameters were observed in either the control or experimental groups. These findings represent an important step toward the development of modern symbiotic feed additives for animals, contributing to the biosafety of domestic poultry farming.

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