Study of post-vaccination immunogenesis in geese in production conditions by the method of immunofermetic analysis

Goose parvovirus (PG) remains a global problem in poultry farming. For this reason, it is necessary to develop a new diagnostic approach that will be simpler and faster than traditional tests, while revealing the breed and age predisposition. We conducted a serological study among geese of different ages of the "Linda" and "Large Gray" breeds for the presence of specific antibodies to the pathogen virus PH after immunization with a live strain of the LIV-22 vaccine. The method of enzyme immunoassay (ELISA) was used to assess the intensity of humoral immunity. Today, ELISA has advantages such as simplicity and speed of work, and also allows you to examine a large number of samples at the same time. However, the method we used has so far been used to determine the level of antibodies in samples without making a relationship between the breed and the age of the bird. Based on the latter, this study has wide practical significance, as it allows for a more detailed study of the nature of the spread of the virus. A series of studies have proven a direct link between the level of antibodies and the age and breed characteristics of the geese. It was found that the older bird shows higher indices of the arithmetic mean titer of antibodies in comparison with the young. The latter allows us to conclude that it is advisable to take a differentiated approach to preventive measures and vaccination, taking into account the characteristics of each specific group of birds, based on their breed and age. The results obtained are the basis for the development of the most optimal vaccination schemes and recommendations for improving the sanitary and hygienic conditions of poultry keeping, contributing to reducing the risk of outbreaks of viral enteritis in geese.

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