Triggers of oxidative stress in the organs of the reproductive system in rats when exposed to deltamethrin

Deltamethrin is widely used in agriculture and veterinary medicine. In recent years, data have begun to appear in the scientific literature on the effect of deltamethrin and other synthetic pyrethroids on the reproductive system and, in particular, on the quality of seminal fluid. However, the mechanisms of development of these disorders are not fully understood. The purpose of this work is to determine the triggers of oxidative stress in reproductive organs of rats exposed to deltamethrin. The study was conducted on 48 male laboratory rats with a body weight of 230-250 g. Metabolic changes in the testes after a single administration of deltamethrin at a dose of 17.4 mg/kg body weight (1/5 LD50) were assessed after 1 and 3 days. The evaluation criteria were the content of pyruvic, lactic and uric acids, inorganic phosphate (Pi), glutathione, malondialdehyde (MDA), the activity of glutathione peroxidase (GPx) and glutathione reductase (GR) in the organs of the reproductive system. The obtained results were subjected to statistical processing. Administration of deltamethrin to laboratory rats at a dose of 1/5 LD 50 causes changes in metabolism in the reproductive organs: testicles, epididymis, prostate gland and seminal vesicles. After 1-3 days, an increase in the content of lactate, pyruvate, urate, Pi, and MDA in the rats' organs was noted. This indicates a violation of energy metabolism and increased lipoperoxidation processes, which leads to a decrease in glutathione levels. Glutathione deficiency is aggravated by the increased activity of antioxidant enzymes (GPx and GR). The trigger mechanism for the development of oxidative stress in the organs of the reproductive system is the disruption of energy metabolism caused by the deep catabolism of purines to uric acid. This process is accompanied by increased generation of free radicals and depletion of the pool of non -enzymatic antioxidants. A decrease in the function of the antioxidant system against the background of MDA accumulation in the organs of the reproductive system indicates the development of oxidative stress.

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