Cytokinetic parameters of erythropoiesis in chickens under the influence of enrofloxacin in experimental salmonellosis

A study was conducted on the effect of enrofloxacin on the cytokinetic parameters of erythropoiesis in chickens of the Highsex Brown cross under conditions of experimental salmonellosis. Enrofloxacin, a member of the fluoroquinolones, is an antibacterial drug with pronounced bactericidal activity against gram-negative microflora, which is widely used in veterinary practice. The aim of the work is to study the effect of enrofloxacin on the cytokinetic parameters of erythropoiesis in the conditions of experimental salmonellosis caused by Salmonella Typhimurium culture. The experiment involved three groups of day-old chicks: intact control, infected control (infection with Salmonella Typhimurium) and the experimental group (infection against the background of the use of enrofloxacin at a dose of 200 mg/l with drinking water for five days). After the drug was discontinued, blood samples were taken on the second and sixth days. The number of reticulocytes was determined by the supravital staining with brilliant cresyl blue. The dynamics of the half-life of reticulocytes, the production of reticulocytes, the half-life of erythrocytes, and the production of erythrocytes were studied. Experimental salmonellosis induced significant disturbances in the kinetics of erythrocyte cell renewal, manifested by an imbalance between the processes of proliferation, differentiation, and elimination of red blood cells. The use of enrofloxacin contributed to the correction of the duration of the half-life of reticulocytes and the imbalance between the production and elimination of erythroid cells. The key result of the study in the experimental group was a significant shortening of the half-life of reticulocytes by the sixth day, which reflects the normalization of the balance between proliferation and differentiation in the erythroid lineage. The use of enrofloxacin demonstrates a protective effect on the erythron system of chickens with salmonellosis, reducing the bacterial load and correcting erythropoiesis disorders. Against the background of therapy, the balance between the production and clearance of erythrocytes is restored.

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