Dynamics of leukogram indicators in sport horses under the influence of a probiotic
https://doi.org/10.52419/issn2072-2419.2026.1.233
Abstract
Probiotics offer advantages over other adaptogens in equestrian sports, as they are administered orally with feed without requiring skin penetration. They also maintain intestinal microflora balance, thereby preventing colic in horses. Furthermore, probiotic use eliminates the risk of using prohibited drugs in sports, unlike herbal dietary supplements. The search for ways to enhance horses' adaptability to physical exercise remains relevant today. The aim of our study was to evaluate the effect of a probiotic on the leukogram of sport horses. Our study examines the use of the probiotic "BonacaAPC" as an adaptogen for Arabian horses participating in endurance racing. The leukogram was calculated by counting the leukocyte forms in blood smears stained with the May-Grünwald method. Leukocyte counting was performed in a counting chamber with a Goryaev grid. In the experimental group, we observed a smaller increase in the relative number of segmented neutrophils in response to physical exercise, as well as a higher relative and absolute number of lymphocytes. Neutrophils and lymphocytes are the most reactive cells, responding most quickly to changes occurring in the body during physical exercise. Based on our research, the probiotic "Bonaca-APK" increased the adaptive capacity of horses, resulting in a less stressful cellular response. Therefore, it can be recommended for use in sport horses as an adaptogen to physical exercise, while also helping to maintain intestinal microflora and reducing the risk of colic, which is also important in equestrian sports.
About the Author
E. K. Velikodnaya
St. Petersburg State University of Veterinary Medicine
Russian Federation
Russian Federation
postgraduate student, assistant of the Department of Biochemistry and Physiology
References
1. Velikodnaya, E. K. The influence of physical activity on the immune-biochemical status of sport horses (literature review) / E. K. Velikodnaya, L. Yu. Karpenko // Current issues in veterinary biology. - 2025. - No. 2 (66). - P. 3-12. - DOI 10.24412/2074-5036-2025-266-3-12. - EDN SQKRQF.
2. The influence of the intestinal microbiome on the performance of athletes in cyclic sports according to ergometric testing indicators / Yu. I. Koryukalov, M. S. Lapshin, M. V. Kondashevskaya [et al.] // Sports medicine: science and practice. - 2024. - Vol. 14, No. 2. - P. 34-44. – DOI 10.47529/2223-2524.2024.2.8. – EDN BPVWTP.
3. Ermakova, I. V. Modern concepts of the mechanisms regulating the function of the hypothalamic-pituitary-adrenal system / I. V. Ermakova // New studies. – 2014. – Vol. 41, No. 4. – P. 77-86.
4. Ermolaeva, E. N. The response of neutrophils to chronic physical activity of varying intensity / E. N. Ermolaeva, S. A. Kantyukov, V. P. Yakovleva // Russian Immunological Journal. – 2018. – Vol. 12, No. 3(21). – P. 286-289. – DOI 10.31857/S102872210002396-0. – EDN VVCGXX.
5. Zinovieva, S. A. Reaction of the body of trotter and heavy draft horses to speedstrength load / S. A. Zinovieva, S. A. Kozlov, S. S. Markin // Horse breeding and equestrian sport. – 2022. – No. 2. – Pp. 17-20. – DOI 10.25727/HS.2022.2.60369. – EDN PYKZQV.
6. Changes in hematological blood parameters in horses participating in endurance riding / B. S. Semenov, V. A. Guseva, T. Sh. Kuznetsova, A. V. Nazarova // International Veterinary Bulletin. – 2022. – No. 3. – P. 193-199. – DOI 10.52419/issn2072-2419.2022.3.193. – EDN ZYKYYD.
7. Immunological parameters of the blood of old horses / M. V. Shchipakin, I. B. Duguchiev, N. V. Zelenevsky [et al.] // Medical Immunology. – 2017. – Vol. 19, No. S. – P. 428. – EDN ZBFHYR.
8. Infectious process / N. P. Chesnokova, V. V. Morrison, G. E. Brill [et al.]. – M.: Publishing house of the Academy of Natural Sciences, 2006. – 484 p.
9. Kozlov, V. A. The Immune System and Physical Activity / V. A. Kozlov, O. T. Kudayeva // Medical Immunology. - 2002. - Vol. 4, No. 3. - Pp. 427-438. – EDN JTOJGR.
10. Clinical Hematology: Textbook / A. A. Aliyev, S. A. Rukavishnikova, T. A. Akhmedov [et al.]. - St. Petersburg: Lan Publishing House, 2021. - 120 p. - ISBN 978-5-8114-7974-0. - EDN VVWSOW.
11. Clinical Diagnostics of Internal NonCommunicable Diseases of Animals / A. M. Smirnov, P. Ya. Konopelko, R. P. Pushkarev [et al.]; ed. M. N. Kurzina. – Moscow.: Agropromizdat, 1988. – 511 p.
12. Markin, S. S. The Role of the Horse in Preserving the Peasantry, Traditional Way of Life, and Human Values of Modern Russian Society / S. S. Markin, S. A. Zinovieva, S. A. Kozlov // Actual Problems of Social and Humanitarian Sciences: A Collection of Articles, Moscow, June 1, 2020. Volume Issue II. – Moscow: Publishing House "Scientific Library", 2020. – Pp. 180-189. – EDN NQJLGX.
13. Leukocyte Morphology as an Indicator of Adaptation of Yakutia Athletes to Intense Physical Activity / E. I. Semenova, L. D. Olesova, Z. N. Krivoshapkina [et al.] // Yakut Medical Journal. – 2014. – No. 2 (46). – P. 80-83. – EDN SQWDIH.
14. Musin, Z. Kh. The human immune system and physical activity / Z. Kh. Musin, S. V. Latukhov // Medical Immunology. – 2007. – Vol. 9, No. 1. – P. 35-38.
15. Evaluation of hematological parameters of rainbow trout when introducing probiotic preparations into the diet / L. Yu. Karpenko, N. A. Sidorova, A. A. Bakhta [et al.] // International Bulletin of Veterinary Medicine. – 2025. – No. 1. – P. 271-277. – DOI 10.52419/issn2072-2419.2025.1.271. – EDN DPUBHG.
16. Petrikeeva, L. V. Homeostasis indicators of purebred Arabian horses during hippodrome trials: specialty 03.03.01 "Physiology": abstract of a dissertation for the degree of candidate of biological sciences / Petrikeeva Lidiya Vladimirovna. - Moscow, 2017. - 22 p. - EDN ZQEGSZ.
17. Use of the probiotic "Biflor" and the immunostimulant "Apistimulin-A" to improve the productivity of broiler chickens / A. A. Glaskovich, L. Yu. Karpenko, A. B. Balykina, A. A. Bakhta // Issues of legal regulation in veterinary medicine. - 2017. - No. 4. - P. 90-92. - EDN ZXWEVJ.
18. Use of a probiotic supplement in pregnant sows under industrial pig farming conditions / A. A. Stekolnikov, L. Yu. Karpenko, N. A. Shinkarevich [et al.] // International Veterinary Bulletin. - 2021. - No. 4. - P. 160-165. - DOI 10.52419/issn2072-2419.2021.4.160. - EDN KDYBXI.
19. Samsonova, A.V. Hormones and hypertrophy of human skeletal muscles: a tutorial / A.V. Samsonova. - St. Petersburg: Kinetika, 2019. - 204 p.
20. Comparative analysis of leukograms obtained by different methods / E. V. Krapivina, D. V. Ivanov, V. A. Sorokina, D. A. Terentyeva // Innovative development of productive and unproductive animal husbandry: Collection of scientific papers of the international scientific and practical conference, Bryansk, May 26-27, 2022. - Bryansk: Bryansk State Agrarian University, 2022. - P. 84-88. - EDN GSUCGD.
21. Shinkarevich, N. A. Analysis of the use of a probiotic feed additive for sows during gestation on the productive indicators of young animals / N. A. Shinkarevich, L. Yu. Karpenko, A. A. Bakhta // Actual problems of treatment and prevention of diseases of young animals: Proceedings of the International scientific and practical conference, Vitebsk, November 2–4, 2022 / Editorial board: N. I. Gavrichenko (editor-in-chief) [et al.]. – Vitebsk: Educational Institution "Vitebsk Order of the Badge of Honor State Academy of Veterinary Medicine", 2022. – pp. 14–16. – EDN
22. MIMMMI.
23. Wilmore, J.H. Physiology of Sport and Physical Activity: trans. from English / J.H. Wilmore, D.L. Costill. - K.: Olympic Literature, 1997. - 504 p.
24. Shinkarevich, N. A. Analysis of the use of a probiotic feed additive to sows during gestation on the productive indicators of young animals / N. A. Shinkarevich, L. Yu. Karpenko, A. A. Bakhta // Actual problems of treatment and prevention of diseases of young animals: Proceedings of the International Scientific and Practical Conference, Vitebsk, November 2-4, 2022 / Editorial Board: N.I. Gavrichenko (editor-in-chief) [et al.]. – Vitebsk: Educational Institution "Vitebsk Order of the Badge of Honor State Academy of Veterinary Medicine", 2022. – P. 14-16. – EDN MIMMMI.
25. Morgado, J.P. Comparison of immunohematological profile between enduranceand power-oriented elite athletes / J.P. Morgado, C.N. Matias, C.P. Monteiro // App. Physiol. Nutr. Metab. – 2017. – 42 (3). – P. 257-262.
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For citations:
Velikodnaya E.K. Dynamics of leukogram indicators in sport horses under the influence of a probiotic. International Journal of Veterinary Medicine. 2026;(1):233-242. (In Russ.) https://doi.org/10.52419/issn2072-2419.2026.1.233
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