Study of the antioxidant effect of cryofractions of the spleen and placenta of cattle

Maintaining the genetic stability of farm animals under adverse environmental conditions is often associated with the state of the antioxidant system and is a pressing issue in veterinary medicine. Our data demonstrate that pharmaceutical substances containing hydrophilic bovine spleen cryofraction (HBSC), lipophilic bovine spleen cryofraction (LBSC), and a mixture of lipophilic bovine spleen and placenta cryofraction (LBSPC) exerted an antimutagenic effect on the animals; however, the mechanism of this effect is far from fully understood. Therefore, the aim of this study was to investigate the effects of HBSC, LBSC, and LBSPC on certain parameters of lipid peroxidation (LPO) and antioxidant defense (AOD) in the blood of rats under conditions of oxidative stress induced by cyclophosphamide (CP). The effects of separate intramuscular administration of pharmaceutical substances at a dose of 0.5 ml/kg and their combined administration with an intraperitoneal injection of CP at a dose of 40 mg/kg in white laboratory male rats were studied. The effects of three doses of HBSC, LBSC, and LBSPC administered 24 hours apart before the CP injection were also studied. A single administration of HBSC, LBSC, and LBSPC did not induce statistically significant changes in lipid peroxidation and antioxidant protection in the blood of rats. A course of HBSC, LBSC, and LBSPC administered to rats reduced oxidative stress induced by CP due to its effect on antioxidant enzymes. This was reflected in a significant (more than 2-fold) increase in catalase activity, as well as glutathione peroxidase activity (by 71-79%), and a decrease in the levels of intracellular reactive oxygen species, diene conjugates, and ketodienes relative to animals in the positive control group. Thus, HBSC, LBSC, and LBSPC possessed an antioxidant effect, manifested by modulating the activity of the enzymatic component of the antioxidant system.

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