Analysis of the results of microbiological studies in case of finger crumb ulcer in cattle
https://doi.org/10.52419/issn2072-2419.2026.1.437
Abstract
Lesions of the distal extremities in lactating cows can be of both infectious and non-infectious etiology. Finger crumb ulcer or finger dermatitis or Mortellaro's disease and necrobacteriosis are infectious diseases. These diseases can occur if zoogynical rules of maintenance are not followed, if preventive measures are not taken (for example, untimely and poor manure harvesting, absence or improper use of preventive baths). Bacteriological material was taken from sick animals in the area of the finger crumb from five animals. The animal was fixed in a special orthopedic machine. Before taking the biopsy, local anesthesia was performed with a 2% solution of novocaine. The lesion was washed with tap water and dried with sterile wipes. The biopsy was performed at the border of healthy and damaged tissue with a tubular scalpel (punch) with a diameter of 5 mm. The samples were placed in tubes with liquid Ames transport medium. Under isothermal conditions, they were transported to the laboratory for 3 hours. Thus, when studying the culture of biopsies of bovine hoof crumb, a variety of microflora (environment, mucous membranes and skin, potentially pathogenic and purulent microflora, opportunistic fungi) and fungi that can potentially participate in the development and maintenance of inflammation of soft tissues, skin, its appendages and subcutaneous fat were isolated. It can be assumed that the main penetration of microorganisms into the focus of inflammation occurs from the external environment, during pathogenesis, the pathological process is complicated by purulent microflora, which further aggravates and complicates the course of the disease.
About the Author
I. V. NenashevRussian Federation
Candidate of Sciences. Veterinary Sciences, Associate Professor of the Department. Anatomy of Obstetrics and Surgery
References
1. Komarov, V.Y. Lameness in cows. / V.Y. Komarov //Bulletin of Agrarian Science. – 2021. – №. 6 (93). – Pp. 25-31. Access mode: https://reader.lanbook.com/journalArticle/624256
2. Simonov, Yu.I. Factors affecting the health of hooves in cows. /Yu.I. Simonov, L.N. Simonova, V.V. Chernenok //Bulletinof the Kursk State Agricultural Academy. – 2018. – №. 3. – Pp. 99-101.Access mode: https://www.elibrary.ru/item.asp?id=35016571
3. Kontsevaya, S.Y. Assessment of the degree of lameness in cows. /S.Y. Kontsevaya, A.M. Nefedov, V.I. Lutsai, et al.//Agrarian Science. – 2022. – Vol. 354. – No. 11-12. – pp. 43-45. Access mode: https://www.elibrary.ru/item.asp?id=47537896
4. Moreira T. F. et al. Risk factors associate ed with lameness and hoof lesions in pasture -based dairy cattle systems in southeast Brazil //Journal of dairy science. – 2019. – Vol. 102. – No. 11. – pp. 10369-10378. Access mode: https://www.sciencedirect.com/science/article/pii/S0022030219307696
5. Passos L. T. et al. Dairy cows change locomotion score and sensitivity to pain with trimming and infectious or non-infectious diseases //Tropical animal health and production. – 2017. – Vol. 49. – No. 4. – pp. 851-856. Access mode: researchgate.net/publication/304357256
6. Janssen S. et al. Pilot study on hormonal, metabolic, and behavioral stress response to treatment of claw horn diseases in acutely lame dairy cows //Journal of Dairy Science. – 2016. – Vol. 99. – No. 9. – pp. 7481-7488. Access mode: DOI: 10.1007/s11250-017-1273-0
7. Proudfoot K. L., Weary D. M., Von Keyserlingk M.A.G. Behavior during transition differences for cows diagnosed with claw horn lesions in mid lactation //Journal of dairy science. – 2010. – Vol. 93. – No. 9. – pp. 3970-3978. Access mode: https://www.sciencedirect.com/science/article/pii/S0022030218307288
8. Whay H. R., Shearer J. K. The impact of lameness on welfare of the dairy cow // Veterinary Clinics: Food Animal Practice. – 2017. – Vol. 33. – No. 2. – pp. 153-164. Available at: DOI: 10.1016/j.cvfa.2017.02.008
9. Lattice M.B. The spread of mastitis in cows and the development of a means of preventing mastitis during the dry season. / M.B. Lattice //Polythematic online electronic scientific journal of the Kuban State Agrarian University. 2013. No. 88. pp. 826-840. Access mode: https://www.elibrary.ru/item.asp?id=26632201
10. Simonov, P.G. The spread of gynecological diseases in cows in the Altai TerritoP.G. Simonov, N.M. Semenikhina // Agricultural science is dedicated to agriculture. 2016. pp. 282-284. Access mode: https://www.elibrary.ru/item.asp?id=26072134
11. Charfeddine N., Pérez-Cabal M. A. The effect of claw disorders on milk production, fertility, and longevity, and their economic impact in Spanish Holstein cows //Journal of dairy science. – 2017. – Vol. 100. – No. 1. – pp. 653-665. Access mode: https://www.sciencedirect.com/science/article/pii/S0022030216307962
12. Bell N. J. et al. The development, implementation and testing of a lameness control program based on HACCP principles and designed for heifers on dairy farms //The Veterinary Journal. – 2009. – Vol. 180. – No. 2. – pp. 178-188. Available at: DOI: 10.1016/j.tvjl.2008.05.020
13. Cramer G. et al. Herd-and cow-level prevalence of foot diseases in Ontario dairy cattle //Journal of dairy science. – 2008. – Vol. 91. – No. 10. – pp. 3888-3895. Access mode: https://www.sciencedirect.com/science/article/pii/S0022030208710154
14. Nenashev, I.V. The prevalence of orthopedic diseases of the distal extremities in cattle, depending on the conditions of detention /I.V. Nenashev, E.M. Maryin // Proceedings of the Samara State Agricultural Academy. 2024. No. 4. pp. 103-108. Access mode: https://www.elibrary.ru/item.asp?id=74146301
15. Manske T., Hultgren J., Bergsten C. Prevention and interrelationships of hoof diseases and lameness in Swedish dairy cows // Preventive veterinary medicine. – 2002. – Vol. 54. – No. 3. – pp. 247-263. Access mode: DOI:10.1016/S0167-5877(02)00018-1
16. Somers J. et al. The prevalence of claw disorders in Dutch dairy cows exposed to several floor systems //Journal of dairy science. – 2003. – Vol. 86. – No. 6. – pp. 2082-2093. Access mode: https://www.sciencedirect.com/science/article/pii/S0022030203737977
17. Bicalho R. C., Machado V. S., Caixeta L. S. Lameness in dairy cattle: A debilitating disease or a disease of debilitated cattle? A cross-sectional study of lameness prevalence and thickness of the digital cushion //Journal of dairy science. – 2009. – Vol. 92. – No. 7. – pp. 3175-3184. Access mode: https://www.sciencedirect.com/science/article/pii/S0022030209706344
18. Green L. E. et al. Temporal associations between low body condition, lameness and milk yield in a UK dairy herd //Preventive veterinary medicine. – 2014. – Vol. 113. – No. 1. – pp. 63-71. Access mode: https://www.sciencedirect.com/science/article/pii/S0167587713003036
19. Griffiths B. E., Grove White D., Oikonomou G. A cross-sectional study into the prevalence of dairy cattle lameness and associated herd-level risk factors in England and Wales //Frontiers in veterinary science. – 2018. – Vol. 5. – p. 65. Access mode: DOI: 10.3389/fvets.2018.00065
20. Oliveira V. H. S., Sørensen J. T., Thomsen P. T. Associations between biosecurity practices and bovine digital dermatitis in Danish dairy herds //Journal of dairy science. – 2017. – Vol. 100. – No. 10. – pp. 8398-8408. Available at: DOI: 10.3168/jds.2017-12815
21. Palmer M. A., O'Connell N. E. Digital dermatitis in dairy cows: A review of risk factors and potential sources of betweenanimal variation in susceptibility //Animals. – 2015. – Vol. 5. – No. 3. – pp. 512-535. Access mode: DOI: 10.3390/ani5030369
22. Simonova, E.Y. Diseases of hooves in cows and pathogenic microflora in necrotic areas. / E.Y. Simonova, A.A. Samokhina // Intensity and competitiveness of livestock industries: mater. National scientific- practical conference, dedication. 85th anniversary of the birth of Honored Worker of Higher Education of the Russian Federation, Honorary Worker of Higher Professional Education of the Russian Federation, Doctor of Biological Sciences, Professor Egor Pavlovich Vashchekin, Bryansk, January 25, 2018. Bryansk: Publishing House of the Bryansk State Agrarian University, 2018. pp. 37-40. Access mode: https://www.elibrary.ru/item.asp?id=35150899
23. Masalov, V.N. Identification of conditionally pathogenic microflora of wound surface flushes in diseases of the distal extremities, cows /V.N. Masalov, V.V. Kreis, S.A. Skrebnev, K.S. Skrebneva //Bulletin of Agrarian Science. 2022. No. 4 (97). pp. 43-46. Access mode: https://www.elibrary.ru/item.asp?id=49455882
24. Rukol, V.M. Investigation of the microbiological composition of exudate from foci of purulent-necrotic skin lesions of the distal extremities of cattle. /V.M. Rukol, O.L. Dubinina //Scientific notes of the educational institution "Vitebsk Order "Badge of Honor" State Academy of Veterinary Medicine". 2013. Vol. 49. No. 2-2. pp. 102-105. Access mode: https://www.elibrary.ru/item.asp?id=21483132
25. Khuzin, D.A. The role of saprophytic and opportunistic microorganisms in the occurrence and spread of opportunistic infections in cattle / D.A. Khuzin, S.A. Yusupov, A.I. Eroshin, E.Y. Tarasova, R.M. Potekhina, A.M. Tremasova //Scientific notes of the Kazan State Academy of Veterinary Medicine named after NE Bauman. – 2022. – Vol. 252. – No. 4. – pp. 267-272. Access mode: https://www.elibrary.ru/item.asp?id=49843434
26. Nadezhdin D.N., Results of bacteriological examination of flushes from purulentnecrotic lesions of hooves in cattle / D.N. Nadezhdin, E.M. Maryin, O.N. Maryina, L.P. Pulcherovskaya, I.F. Kalimullin. // Proceedings of the Orenburg State Agrarian University. 2025. No. 1 (111). pp. 151-158. Access mode: https://www.elibrary.ru/item.asp?id=80372393
27. Maryin, E.M. Indication of microorganisms in purulent-necrotic lesions of the skin base in the hoof area in cows by PCR /E.M. Maryin, V.A. Ermolaev //Bulletin of the Ulyanovsk State Agricultural Academy. – 2016. – №. 4 (36). – Pp. 135-139. Access mode: https://www.elibrary.ru/item.asp?id=2763279236). – Pp. 135-139. Access mode: https://www.elibrary.ru/item.asp?id=27632792
Review
For citations:
Nenashev I.V. Analysis of the results of microbiological studies in case of finger crumb ulcer in cattle. International Journal of Veterinary Medicine. 2026;(1):437-447. (In Russ.) https://doi.org/10.52419/issn2072-2419.2026.1.437
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