Dynamics of red blood under femoral bone fractures in rats on the background of application of rv-2 immunomodulator and rvi biocompositional material

Abstract

The paper presents data on the dynamics of some indicators of red blood in outbred white rats during osteosynthesis of tubular bones on the background of the application of the RVI biocomposite material preparation and the RV-2 immunomodulator from the group of synthetic dipeptides. The experiments were performed on 30 animals, divided into six groups (n=6in each). Therat softhecontrol group after osteo-synthesiswerenottreated (untreated wound model), experimental rats from the 1st, 2nd, 3rd, 4th and 5 th groups were treated with the preparations separately or in combination at various times after surgery (immediately or 5 daysafter). Blood for the study was sampled before surgery, and then with a weekly interval. The duration of the experiment was 21 days. It was found that the indicators of red blood in animals of all groups were within the physiological norm. On the 7th and 14th day, a decrease in the number of erythrocytes (with the exception of the 4th and 5th groups, respectively), hemoglobin, hematocrit (7th day) and an increase in the width of the distribution of the erythrocyte population were noted. All-therest hematological parameters were multidirectional. By the end of the experiment, the amount of hemoglobin, its average content and concentration in red blood cells, the width of the distribution of red blood cell populations in all groups exceeded the background indicators. At the same time, hematocrit level and mean red blood cell volume were lower. The number of erythrocytes exceeded the initial level only in the 2nd and 3rd groups by 2.6 and 3.7%, respectively, where the animals were treated with RV-2. Relatively stable hematological parameters for the entire period of the experiment indicate a high resistance of outbred rats to injuries. It can be assumed that the use of the RV-2 preparation has a stimulating effect on erythropoiesis in varying degrees depending on the timing of its use at different stages of reparative osteogenesis. The stimulating effect of biocomposite material on bone tissue regeneration is probably due to increased tissue metabolism.