Some aspects of the local immune response in breast tissue

Abstract

Currently, mastitis in cattle is widespread, as a result of which cattle farms are inflicted colossal economic damage due to a decrease in milk yield and in milk quality, culling of cows, an increase in the incidence of calves and the cost of their treatment. Another, no less important problem is the presence of inhibitory substances in milk during the treatment of animals when using complex anti-mastitis preparations containing combinations of various groups of antibiotic substances. In connection with the high resistance of mastitis pathogens to the action of antibiotics, antigenic stimulation methods are becoming increasingly important in the fight against mastitis. This article discusses the formation of a local immune response in the mammary glands of experimental rats. During the study, we noted an increase in the number of antigen presenting cells in mice of the experimental group compared to the control. Cells were counted by light microscopy in a hundred fields of view. At the same time, the average number of macrophages in one hundred fields of vision in the experimental group is 70, and in the control group 40, which is almost two times less. Thus, it can be concluded that the number of tissue macrophages increases as a result of exposure to the vaccine, which indicates their immune activity. Our researches allow us to reveal some aspects of the mechanism of action of the studied method of treating mastitis, which opens further prospects for recommending it for mass use in veterinary practice, since the number of tissue macrophages in immunized mice of the experimental group significantly exceeds the number of macrophages in untreated mice of the control group.