Rational biotechnological justification and tendencies of manufacture and application of inactivated colisalmonellosis vaccine for pigs

Due to the fact that currently existing methods of specific prophylaxis are not always effective, the incidence and mortality from escherichiosis remains high and is recorded in many farms of the Russian Federation. For immunotherapy and prevention of intestinal infections in young animals, 4 types of biologics are used: vaccines that create active immunity; immune sera and immunoglobulins; immunostimulants; bacteriophages. For the early prevention of infectious diseases, the most effective is vaccination. The purpose of the work is to develop the technological regulations for the manufacture, testing, study of the biocidal and therapeutic properties of colisalmonella anatoxin vaccine. Technological regulations have been developed for the preparation and use of colisalmonella anatoxin vaccine. The use of synthetic nutrient medium for the separate cultivation of Salmonella and Escherichia coli with a concentration of microorganisms up to 7-9x1010 mx. / ml, providing a complete and irreversible detoxification of a complex of Escherichia coli and Salmonella endo- and ekzotoxins by autoclavation them with two detoxicants: in the first stage 0.2 % formalin solution (instead of 0.8%) at 40 ° C for 7 days, in the second stage - with a 0.5% solution of etonium at 41 ± 1 ° C for 7 days, allowed us to obtain an effective and harmless biological product - anatoxin-vaccine. Testing of colisalmonella anatoxin-vaccine was carried out on the basis of pig-breeding complexes of Kursk and Kaluga regions. Colisalmonella toxoid vaccine was sucked up to piglets twice at a dose of 35-50 ml / head for 2-3 days. Revaccination was performed after 2-3 weeks. An increase in the therapeutic and prophylactic effect of the experimental colisalmonella anatoxin vaccine is achieved by additional feeding of probiotic microorganisms (lactic acid bacteria) and chelate compounds of micro- and macroelements 5-6 hours after each administration of the experimental vaccine.

колисальмонеллезная анатоксин-вакцина, кишечная палочка, сальмонеллы, глутаровый альдегид, этоний, ионы серебра, пробиотики, хелаты, colisalmonella anatoxin -vaccine, E. coli, salmonella, glutaraldehyde, etonium, silver ions, probiotics, chelates

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