Isolation of antimicrobial peptides from larvae of Hermetia illucens and the prospect of their applying

Nowadays antimicrobial peptides (AMPs) are considered by researchers as a promising object for development of a new generation of antibacterial drugs. AMPs are insect innate immunity molecules that, during evolution, provide effective protection against various infectious agents. The benefits of AMP include their activity against a wide range of microorganisms (bacteria, viruses, fungi), fast killing effect, a potentially low probability of resistance, the accompanying spectrum of anti-inflammatory properties. The widespread administration of antibiotics as antimicrobial drugs resulted in the existance of antibiotic-resistant strains. Therefore, the search for new promising antibacterial agents of biological origin can be a prerequisite for the development of highly effective antimicrobial agents that can replace traditional drugs. The aim of the research was to isolate antimicrobial peptides from the biomass of Hermetia illucens larvae and to study their antimicrobial activity. To achieve this, it was necessary to solve the following tasks: isolate AMP from the biomass of larvae, separate protein fractions by high performance liquid chromatography and to study their antimicrobial activity. As a result of the experiment, 7 protein fractions from the biomass of Hermetia illucens larvae were obtained. The antimicrobial activity of the isolated proteins fractions were studied. The antimicrobial activity of two fractions, N 4 and 7, were studied in the first case against Candida albicans str. РКПГY-401 / NCTC-885-653, Staphylococcus aureus str. ATCC 6538 (209-P), Salmonella typhimuriumstr. 1626, in the second case against Bacillus cereus str. ATCC 11778, Candida albicans str. РКПГY-401 / NCTC-885-653, Staphylococcus aureus str. ATCC 6538 (209-P), Salmonella typhimurium str. 1626.

антимикробные пептиды, антимикробная активность, Candida albicans, Staphylococcus aureus, Salmonella typhimurium, Bacillus cereus, antimicrobial peptides, antimicrobial activity, Candida albicans, Staphylococcus aureus, Salmonella typhimurium, Bacillus cereus

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