Аntimicrobial activity test of the feed additives Eteracide and BiSalTech

Abstract

The aim of this work was to study the antimicrobial activity and compare efficacy of the drugs Eteracid and BiSalTech against the main causative agents of bacterial diseases of animals and birds. 3 cultures of 11 types of pathogens were used in the work: one culture of Salmonella gallinarum-pullorum Salmonella typhimurium, Salmonella group E, Proteus vulgaris, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus citreus, Staphylococcus epidermidis, Streptococcus zooepidemicus; and 2 cultures of Escherichia coli and Salmonella enteritidis (from chickens and quail). Antimicrobial activity of additives were determined by the method of serial dilutions. Crops incubated in a thermostat at a temperature of 37,5 С 24 hours, and then were removed from MPB to solid media - Endo agar (Escherichia coli, Salmonella enteritidis, Salmonella gallinar-um, Salmonella typhimurium, Salmonella group E), KHLD-agar (Escherichia coli), MPA (Proteus vulgaris, Pseudomonas aeruginosa, Streptococcus zooepidemicus), Staphylococcus agar (Staphylococcus aureus, Staphylococcus citreus, Staphylococcus epidermidis) and the growth of colonies of cultures of pathogens were calculated. The minimum inhibitory concentration (MPC) was determined by the lowest concentration of the drug, which suppressed the visible growth of the test microorganism. The minimum bactericidal concentration (MBC) was determined by the lowest concentration of the drug, at which there were no growth of microorganisms in solid media. The results are reflected in the calculation of the effective dose of the drug at the rate of one liter of the drug per ton of drinking water.