The effect of methods of administration biotinylated oxidized dextran derivative on the indices of cellular immunity of laboratory animals

The effectiveness of each pharmacological composition based on dextran depends on the average size of its molecules. Therefore, oxidized dextrans are of particular interest for pharmacology, since they have a high biocompatibility with the body and do not exhibit allergenic properties. Studies were carried out to study the effect of biotinylated oxidized dextran derivative (AML) on the cellular immunity of laboratory animals. The experiments were carried out at the Institute of Experimental Veterinary Medicine of Cibiri and the Far East of the FSCA RAS. The biotinylated derivative of oxidized dextran (ROD) manufactured by Federal Research and Production Center Altai (Federal Research and Production Center Al-Thai) was used as an object of research. Researches were conducted for the purpose of studying of influence of the biotinylated oxidized dextran derivative (BOD) on cellular immunity laboratory animals. Experiments are made at Institute of the experimental veterinary medicine of Siberia and the Far East SFNCA RAN. For statement of experiments by the principle of analogs created 4 groups (n=15) of white mice. To mice of all skilled groups entered BOD in a dose of 0,1 ml (5mg/kg alive weight). To the first experienced group BOD entered intramusculary, the second - subcutaneously, the third - it is oral. Mice of the fourth group served as monitoring, medicine wasn't given them. The maximum increase in number of cages in charge of cell-like immunity was observed in the first skilled group of mice to whom BOD entered in a dose 5 mg/kg of alive mass of an animal intramusculary, fivefold every 48 hours. The number of monocytes at animals increased by 58,33% and 44,0% (0,38x109/l and 0.36x109/l), and granulocytes - for 31,19% and 32,26% (2,86x109/l and 2,87 x109/l) respectively in comparison with animals of control group.

клеточный иммунитет, лабораторные животные, окисленный декстран, фагоцитарная активность, cellular immunity, laboratory animals, oxidized dextran, phagocytic activity

1. Иммунология / E.C. Воронин, А.М. Петров, М.М. Серых, Д.А. Девришов ; под ред. Е.С. Воронина. - Москва : Колос-Пресс, 2002. - 408 с.

2. Метод комплексной оценки фагоцитарной активности нейтрофилов крови : инструкция по применению / А.М. Горчаков, Н.Г. Кручинский, Ф.Т. Горчакова, И.Н. Коростелева ; НИИЭиПП, Беларусь. - 2003. - 15 с.

3. Молекулярно-массовое фракционирование окисленного декстрана / Д.Ю. Глазев, А.С. Жарков, А.В. Фролов [и др.] // Вестн. Алтайской науки. - 2015. - № 2. - С. 11-13.

4. Ройт, А. Иммунология : пер. с англ. / А. Ройт, Дж. Бростофф, Д. Мейл. -Москва : Мир, 2000. - 592 с.

5. Шкурупий, В.А. Лизосмотропизм - проблемы клеточной физиологии и медицины / В.А. Шкурупий, Ю.Н. Курунов, Н.Н. Яковченко. - Новосибирск, 1999.-288 с.

6. Шкурупий, В.А. Туберкулезный гранулематоз. Цитофизиология и адресная терапия / В.А. Шкурупий ; Рос. акад. мед. наук. - Москва : Изд-во РАМН, 2007. - 536 с.