Determination of chronic toxicity and allergenic properties of the new anti-mastitis drug "Alvesol"

The aim of this study was to investigate chronic toxicity and determination of allergenic properties of new anti-mastitis preparation "Alvesol". Chronic toxicity studies were performed on 3 experimental and 1th control groups of Wistar rats. To determine the allergenic properties of the drug used the method of cutaneous applications and conjunctival test on rabbits of the breed "Chinchilla" of both sexes.During the study, hematologic and biochemical blood indices were determined, physiological parameters (ECG, HR, HR, "Open field" test, daily diuresis, general urine analysis). Pathomorpho-logical examination was performed on 30% of the animals of each group 14 days after the last administration of the preparation. During clinical examination of animals of the experimental and control groups, the skin and mucous membranes had a natural color. According to the results of these dynamics of live weight, it was noted that the animals of the experimental groups had a high growth rate in comparison with the control one. The indicators for periodic thermometry were within the reference physiological norm of 37.5-39.0 ° C. During the experiment, no death of laboratory rats was recorded. In the study of allergic properties of the drug for the entire period of the skin, there were no irritations, erythemum, infiltration. The results of the conjunctival test showed that the number of reacted animals is less than 50% of the group, and the observed effect is considered as a manifestation of the individual sensitivity of the drug, according to which the "Alvesol" preparation can not be classified as a potential allergen. Based on the complex of studies, it is established that the drug "Alvesol" for long-term use is harmless for warm-blooded animals, does not have toxic properties during long-term use, allergic and local irritant properties are not expressed.

хроническая токсичность, аллергизирующие свойства, противомаститный препарат, доклиническое исследование, chronic toxicity, allergic properties, anti-mastitis drug, pre-clinical study

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