Pharmacological properties of new medicinal gel with chlorhexidine

Abstract

It is expediently to use complex drugs that have a wider spectrum of action and, accordingly, are active against both the original etiologic factor and the secondary microflora, too. The object of the study was a wound healing gel containing 4% chlorhexidine, developed at the Department of Pharmacology and Toxicology of St. Petersburg State Academy of Veterinary Medicine. The gel is a gel-like substance of gray color. It includes chlorhexidine which is stabilized by hydrogel methyl silicic acid. The osmotic activity of the researched drug was studied by dialysis in comparison with a 10% solution of sodium chloride. Investigation of antimicrobial activity was conducted in vitro by well method against to reference strains of microorganisms - the main potential causative agents of wound purulent processes: Escherichia coli (strain 1257), Staphyloccus aureus (strain 906), Pseudomonasa eruginosa, Proteus mirabilis. Incorporation of antimicrobial activity was conducted by measuring the growth retardation zone of microorganisms. The hydration activity of the studied preparations was studied by dialysis through a semipermeable membrane. The amount of the absorbed solution of purified water was measured. The result was expressed as a percentage of the original basis weight. Dialysis was conducted to the level of a constant, unchanging mass of the system under study. The results of the studies showed that the lowest antimicrobial activity was in the gel with 0.05%, the highest with 4% chlorhexidine content. The osmotic activity of this drug was 285%. The sorption effect of 4% gel with chlorhexidine was continued for 15 hours, compared with 10% sodium chloride solution, the osmotic effect of which lasted for 5 hours. High sorption activity, within 14 hours, allows to apply a complex drug to the affected tissue one, twice a day, thereby increasing the economic effect of the treatment.