Influence of the phytobiothic complex on laboratory animals

Abstract

Studies of acute and subacute toxicity of the new phytobiotic sorption complex were carried out on the basis of the St. Petersburg Academy of Veterinary Medicine. The study of the studied parameters was studied according to the "Guidelines for conducting preclinical studies of medicines", ed. A.N. Mironov (2012). The hazard class of the investigational drugs was determined according to GOST 12.1.007-76. To study the parameters of acute toxicity, four groups of rats with 30 heads each were formed. Two experimental groups were injected phyto-biotic complex inside, in a double and fourfold dose, from the experimentally therapeutic 5,800 and 12,000 mg / kg, rats in the control groups were administered a similar amount of isotonic sodium chloride solution. To study the subacute toxicity of the phyto-biotic complex, two groups of white Wistar rats weighing 160 ± 10.0 g were formed, 30 heads each. A phyto-biotic complex at a dose of 6000 mg/ kg was added to the experimental group of laboratory animals for a standard diet for 28 days. The control group received a standard diet, without any additives. The animals were weighed and, at the end of our experiment, conducted a comprehensive survey of the experimental animals. Blood samples were taken to determine hematological and biochemical parameters. An acute experiment on the effect of the phyto-biotic complex on laboratory animals showed that oral administration, in a double and a fourfold dose, from experimentally therapeutic, does not have a toxic effect. The case of experimental animals was not fixed. By the degree of toxicity, the investigated phyto-biotic complex can be classified as the IV hazard class (low-hazard substances).