The use of multiplex PCR-RT in molecular diagnosticsof BLV at perinatal infection
Abstract
The article presents experimental data on the molecular diagnostics of retroviral infection induced by the bovine leukemia virus using the PCR test system developed on the basis of the VIEV RAS multiplex PCR-RT. Circulation of passively acquired colostral antibodies in the body of calves complicates the task of timely diagnosis by serological methods, what makes direct diagnosis of infection at the age of 0-6 months extremely urgent. Based on the results of studies to determine the level of perinatal infection of calves in the dynamics, as part of the implementation and improvement of the scheme of preventive activities in the surveyed farm of the Moscow Region, a tendency to its reduction in 3 times revealed. Using the developed diagnostic test among 710 animals under 3 months of age, the proportion of calves-virus carriers was 6.4%. The possibility of effec tive application of the developed oligonucleotides for DNA detection of BLV by the PCR-RT method for perinatal infection in calves in the early stages of the disease shows in the process of working out the technique on the presented material.
About the Author
N. G. Kozyreva
Federal State Budgetary Research Institution «Federal Scientific Center - All-Russian Scientific Research Institute Of Experimental Veterinary Of The Russian Academy Of Sciences»
Russian Federation
Russian Federation
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Review
For citations:
Kozyreva N.G. The use of multiplex PCR-RT in molecular diagnosticsof BLV at perinatal infection. International Journal of Veterinary Medicine. 2018;(4):28-32. (In Russ.)
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